缺氧条件下水溶液中聚尿苷酸（poly(U)）中氢原子诱导链断裂的速率及速率决定步骤
Rate and rate-determining step of hydrogen-atom-induced strand breakage in poly(U) in aqueous solution under anoxic conditions.
作者信息
Bothe E, Selbach H
出版信息
Z Naturforsch C Biosci. 1985 Mar-Apr;40(3-4):247-53. doi: 10.1515/znc-1985-3-419.
The rate constant for strand breakage in poly(U) after reaction with hydrogen atoms in deoxygenated aqueous solution has been determined to be k = 1.5 s-1 at pH = 4-5 and 24 degrees C. Dithiothreitol has been found to prevent strand break formation by reacting with H-adduct radicals of poly(U) with a rate constant of 5 X 10(6) M-1 s-1. It is concluded that the rate-determining step in H atom-induced strand breakage in poly(U) at pH less than or equal to 6 is the decay of uracil moiety H-adduct radicals via H-abstraction from the ribose moiety.
在脱氧水溶液中，聚（U）与氢原子反应后链断裂的速率常数已确定为：在pH = 4 - 5和24摄氏度时，k = 1.5 s⁻¹。已发现二硫苏糖醇通过与聚（U）的H - 加合物自由基反应来防止链断裂的形成，其反应速率常数为5×10⁶ M⁻¹ s⁻¹。得出的结论是，在pH小于或等于6时，氢原子诱导聚（U）链断裂的速率决定步骤是尿嘧啶部分的H - 加合物自由基通过从核糖部分夺取氢而衰变。
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