Ma Nan, Li Rong, Zhang Gui-Fang, Gao Ruo-Han, Zhang Dong-Jie
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; Heilongjiang Bayi Agricultural University Mudanjiang Institute of Food and Biotechnology, Mudanjiang 157000, China; Daqing Center of Inspection and Testing for Rural Affairs Agricultural Products and Processed Products, Ministry of Agriculture and Rural Affairs, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Natural Product Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea.
Int J Biol Macromol. 2025 May;306(Pt 3):141666. doi: 10.1016/j.ijbiomac.2025.141666. Epub 2025 Mar 1.
Quinoa β-glucan (QBG) has shown potential benefits in treating type 2 diabetes mellitus (T2DM); however, comprehensive evaluations of its effects remain limited. This study investigates the impact of QBG-derived from hot water extraction (Q-) and microbial fermentation enrichment (Q+)-on serum glucose levels, lipid profiles, appetite-regulating hormones, fecal short-chain fatty acids (SCFAs), and gut microbiota composition and function in streptozotocin/high-fat diet (STZ/HFD)-induced T2DM mice. The results indicate that QBG treatment significantly reduced fasting blood glucose, insulin levels, triglycerides (TG) and total cholesterol (TC), while concurrently increasing high-density lipoprotein cholesterol (HDLC) levels. Additionally, liver and pancreatic function improved, as evidenced by decreased levels of malondialdehyde (MDA), aspartate transaminase (AST), and alanine transaminase (ALT). SCFA levels were significantly higher in QBG-treated groups compared to MC group. QBG treatment also reduced the abundance of Firmicutes and Patescibacteria, along with the Firmicutes/Bacteroidota ratio, while increasing levels of Bacteroidota and Actinobacteria. These findings suggest that QBG can regulate the dysbiosis of SCFAs production in T2DM mice and may indirectly modulate the secretion of appetite-regulating hormones by influencing gut microbiota composition. Furthermore, PICRUSt analysis revealed that QBG treatment, particularly Q + _H, could enhance disrupted metabolism and improve gut microbiota functions, helping restore normal physiological function.
藜麦β-葡聚糖（QBG）已显示出在治疗2型糖尿病（T2DM）方面的潜在益处；然而，对其效果的全面评估仍然有限。本研究调查了热水提取法（Q-）和微生物发酵富集法（Q+）获得的QBG对链脲佐菌素/高脂饮食（STZ/HFD）诱导的T2DM小鼠的血糖水平、血脂谱、食欲调节激素、粪便短链脂肪酸（SCFA）以及肠道微生物群组成和功能的影响。结果表明，QBG治疗显著降低了空腹血糖、胰岛素水平、甘油三酯（TG）和总胆固醇（TC），同时提高了高密度脂蛋白胆固醇（HDLC）水平。此外，丙二醛（MDA）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平降低，表明肝脏和胰腺功能得到改善。与MC组相比，QBG治疗组的SCFA水平显著更高。QBG治疗还降低了厚壁菌门和Patescibacteria的丰度以及厚壁菌门/拟杆菌门的比例，同时增加了拟杆菌门和放线菌门的水平。这些发现表明QBG可以调节T2DM小鼠中SCFA产生的失调，并可能通过影响肠道微生物群组成间接调节食欲调节激素的分泌。此外，PICRUSt分析显示，QBG治疗，特别是Q + _H，可以增强紊乱的代谢并改善肠道微生物群功能，有助于恢复正常生理功能。
