Chérif Nadia, Ghedira Kais, Agrebi Houda, Najahi Semah, Mejri Hiba, Azouz Saifeddine, Kielbasa Mélodie, Armengaud Jean, Kangethe Richard Thiga, Wijewardana Viskam, Bouhaouala-Zahar Balkiss, Sghaier Haitham
Disease of Aquatic Organism Service, Aquaculture Laboratory (LR16INSTM03), National Institute of Sea Sciences and Technologies, Salammbô, 2025, Tunisia.
Laboratory of Bioinformatics, Biomathematics and Biostatistics - LR16IPT09, Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis, 1002, Tunisia.
Vet Res Commun. 2025 Mar 4;49(3):125. doi: 10.1007/s11259-025-10688-x.
The analysis of animal responses to immunization is fundamental to vaccine development, enabling the evaluation of immune responses and the elucidation of key mechanisms underlying protective immunity. Such insights are essential for advancing vaccines through clinical trial stages and regulatory approval. Furthermore, understanding the molecular signatures of approved vaccines not only deepens our knowledge of their function but also guides the rational design and improvement of future vaccines. This study aims to elucidate alterations in protein abundance patterns in the sera of European sea bass, D. labrax, following immunization with a chemically-inactivated nodavirus vaccine. The shotgun proteome comparison revealed that in vaccinated animals, compared to controls, there is a modulation of the redox balance favouring reactive oxygen species (ROS), an intricate interaction between coagulation and the immune system resulting in the lower abundance of hematopoiesis-related FLT3, and indications of functional adaptive immunity demonstrated by the lower abundance of pentraxin fusion protein-like and the over-representation of myosins. To the best of our knowledge, this study represents the inaugural investigation of the immune response in fish using a proteomics approach, employing D. labrax as the host and nodavirus as the pathogen used for vaccination and challenge.
分析动物对免疫接种的反应是疫苗开发的基础，有助于评估免疫反应并阐明保护性免疫的关键机制。这些见解对于推动疫苗进入临床试验阶段和获得监管批准至关重要。此外，了解已批准疫苗的分子特征不仅能加深我们对其功能的认识，还能指导未来疫苗的合理设计和改进。本研究旨在阐明欧洲海鲈（D. labrax）在用化学灭活诺达病毒疫苗免疫后血清中蛋白质丰度模式的变化。鸟枪法蛋白质组比较显示，与对照相比，接种疫苗的动物体内氧化还原平衡向有利于活性氧（ROS）的方向调节，凝血与免疫系统之间存在复杂相互作用，导致造血相关的FLT3丰度降低，并且通过五聚体融合蛋白样丰度降低和肌球蛋白过度表达表明存在功能性适应性免疫。据我们所知，本研究是首次使用蛋白质组学方法对鱼类免疫反应进行的研究，以D. labrax作为宿主，诺达病毒作为用于免疫接种和攻毒的病原体。
