Olson R J, McMain M E, Slappey T E
Ann Ophthalmol. 1979 Dec;11(12):1875-8.
One hundred twenty-seven cultures were taken from donor eyes in McCarey Kaufman (M-K) media and 60 from anterior chamber cultures of whole eyes. From the 127 eyes, 17 positive culture were obtained, 13 of which came from anterior chamber cultures of the whole eye and 4 from the M-K media. Of the 16 eyes used for corneal transplantation, there was 1 successfully treated clinical corneal infection. It is concluded that patients with chronic debilitating disease possibly have terminal bacteremia and donor contamination. It is our current recommendations that all corneas obtained in tissue culture media be cultured by the eye bank upon arrival and that eyes prepared for M-K media and refrigerated whole eyes have an anterior chamber culture. Any positive results should then be sent to the primary surgeon as soon as the culture results are ascertained.
在麦凯里·考夫曼（M-K）培养基中从供体眼中采集了127份培养物，从全眼前房培养物中采集了60份。在这127只眼中，获得了17份阳性培养物，其中13份来自全眼前房培养物，4份来自M-K培养基。在用于角膜移植的16只眼中，有1例临床角膜感染得到成功治疗。得出的结论是，患有慢性衰弱性疾病的患者可能存在终末期菌血症和供体污染。我们目前的建议是，组织培养基中获得的所有角膜在到达眼库时都应由眼库进行培养，准备用于M-K培养基的眼和冷藏的全眼应进行前房培养。一旦确定培养结果，任何阳性结果都应立即发送给主刀医生。
