Sakoda Kazuma, Sakurai Atsushi, Imamura Sousuke
Space Environment and Energy Laboratories, NTT Corporation, Tokyo, Japan.
Front Plant Sci. 2025 Feb 18;16:1532522. doi: 10.3389/fpls.2025.1532522. eCollection 2025.
Although photosynthetic response to light has been extensively studied at the single-leaf level, little is known about the response at the whole-plant level. The present study aims to reveal the differences in the photosynthetic response to light under steady and non-steady states between the single leaf and whole plant in and to investigate the mechanisms underlying these differences with respect to leaf aging. First, we developed an open system for gas exchange measurement of the whole plant of Arabidopsis. It enabled the photosynthetic response to dynamic environmental changes to be directly compared between the single leaf and whole plant. The photosynthetic response to the fluctuating light did not differ significantly between the single leaf and whole plant. This result is partly confirmed by the fact that the leaves at different ages showed no difference in the photosynthetic induction after a step change in light. On the other hand, light response analysis for steady-state photosynthesis showed a higher apparent quantum yield in the whole plant than in the single leaf. This difference might be attributed to the difference in the efficiency of light absorption and/or utilization of absorbed light among the leaves at different ages.
尽管在单叶水平上对光合对光的响应已进行了广泛研究，但对于整株植物水平的响应却知之甚少。本研究旨在揭示拟南芥单叶和整株植物在稳态和非稳态下光合对光的响应差异，并研究这些差异与叶片衰老相关的潜在机制。首先，我们开发了一个用于测量拟南芥整株植物气体交换的开放系统。它能够直接比较单叶和整株植物对动态环境变化的光合响应。单叶和整株植物对波动光的光合响应没有显著差异。不同年龄叶片在光阶跃变化后的光合诱导没有差异这一事实部分证实了该结果。另一方面，对稳态光合作用的光响应分析表明，整株植物的表观量子产率高于单叶。这种差异可能归因于不同年龄叶片之间光吸收效率和/或吸收光利用效率的差异。
