Monticello D J, Bakker D, Finnerty W R
Appl Environ Microbiol. 1985 Apr;49(4):756-60. doi: 10.1128/aem.49.4.756-760.1985.
The microbial transformation of dibenzothiophene (DBT) is of interest in the potential desulfurization of oil. We isolated three soil Pseudomonas species which oxidized DBT to characteristic water-soluble, sulfur-containing products. Two of our isolates harbored a 55-megadalton plasmid; growth in the presence of novobiocin resulted in both loss of the plasmid and loss of the ability to oxidize DBT. Reintroduction of the plasmid restored the ability to oxidize DBT to water-soluble products. The products resulting from the oxidation of DBT were characterized and included 3-hydroxy-2-formyl benzothiophene, 3-oxo-[3'-hydroxy-thionaphthenyl-(2)-methylene]-dihydrothionaph thene, and the hemiacetal and trans forms of 4-[2-(3-hydroxy)-thianaphthenyl]-2-oxo-3-butenoic acid. The products of DBT oxidation were inhibitory to cell growth and further DBT oxidation. DBT oxidation in our soil isolates was induced by naphthalene or salicylate and to a much lesser extent by DBT and was repressed by succinate.
二苯并噻吩（DBT）的微生物转化在石油潜在脱硫方面备受关注。我们从土壤中分离出三种假单胞菌，它们可将DBT氧化为具有特征性的水溶性含硫产物。我们分离出的菌株中有两株含有一个55兆道尔顿的质粒；在新生霉素存在的情况下生长会导致质粒丢失以及氧化DBT的能力丧失。重新导入该质粒可恢复将DBT氧化为水溶性产物的能力。对DBT氧化产生的产物进行了表征，包括3-羟基-2-甲酰基苯并噻吩、3-氧代-[3'-羟基-硫茚基-(2)-亚甲基]-二氢硫茚，以及4-[2-(3-羟基)-硫茚基]-2-氧代-3-丁烯酸的半缩醛和反式形式。DBT氧化产物对细胞生长和进一步的DBT氧化具有抑制作用。我们分离出的土壤菌株中，DBT氧化由萘或水杨酸盐诱导，由DBT诱导的程度要小得多，并且会被琥珀酸盐抑制。
