Yosida T H
Gan To Kagaku Ryoho. 1985 Jun;12(6):1348-55.
Cytogenetical technique newly developed for the observation of chromosomes, such as differential staining of sister-chromatids the high-resolution banding and in situ molecular hybridization are introduced in this chapter. In the differential sister-chromatid staining, acricine-orange, BUdR and FPG techniques are described. In the high resolution banding, amethopterin, BUdR and ethidium methods are introduced. RNA/DNA and DNA/DNA in situ hybridization techniques are also included.
本章介绍了新开发的用于观察染色体的细胞遗传学技术，如姐妹染色单体的差异染色、高分辨率显带和原位分子杂交。在姐妹染色单体差异染色中，描述了吖啶橙、5-溴脱氧尿嘧啶核苷（BUdR）和荧光偏振光（FPG）技术。在高分辨率显带中，介绍了氨甲蝶呤、BUdR和溴化乙锭方法。还包括RNA/DNA和DNA/DNA原位杂交技术。
