[Development of technics for observation of chromosomes by the differential staining of sister chromatids, high resolution banding and the in situ molecular hybridization].

Cytogenetical technique newly developed for the observation of chromosomes, such as differential staining of sister-chromatids the high-resolution banding and in situ molecular hybridization are introduced in this chapter. In the differential sister-chromatid staining, acricine-orange, BUdR and FPG techniques are described. In the high resolution banding, amethopterin, BUdR and ethidium methods are introduced. RNA/DNA and DNA/DNA in situ hybridization techniques are also included.