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基于网络药理学、分子对接及实验验证解析复方紫癜汤治疗过敏性紫癜的药理机制

Deciphering the Pharmacological Mechanism of Compound Purpura Decoction in Treating Henoch-Schonlein Purpura by Network Pharmacology, Molecular Docking and Experimental Validation.

作者信息

Su Rong, Ma Quanwu, Zhao Yuna, He Yanping, Yu Na, Lei Yaya, Wang Ziyan, Chen Jing, Gao Ting

机构信息

Department of Pharmaceutical Preparation, General Hospital of Ningxia Medical University, Yinchuan, China.

出版信息

Chem Biodivers. 2025 Jul;22(7):e202402793. doi: 10.1002/cbdv.202402793. Epub 2025 Mar 18.

DOI:10.1002/cbdv.202402793
PMID:40045447
Abstract

Henoch-Schonlein purpura (HSP) is an immunoglobulin A (IgA)-mediated systemic vasculitis that frequently develops in children and may progress to serious complications. Compound purpura decoction (CPD), a classical herbal combination, exerts favourable effects on the clinical symptoms and prognosis of HSP; however, the underlying molecular mechanism remains unclear. First, high-performance liquid chromatography analysis identified five bioactive components in CPD, including protocatechuic acid, chlorogenic acid, mangiferin, baicalin and buddleoside, with contents of 0.011 ± 0.02, 0.577 ± 0.33, 0.150 ± 0.05, 1.132 ± 0.23 and 0.369 ± 0.23 mg/g, respectively. Additionally, we established an animal model of allergic purpura to evaluate the therapeutic effects of CPD on HSP. CPD effectively alleviated renal and cutaneous vasculitis and reduced IgA deposition. CPD could regulate the Treg/Th17 cell balance, decrease the levels of the proinflammatory factors interleukin-6 and tumour necrosis factor-α, and suppress the expression of C3 and C5 (p < 0.05). Network pharmacology analysis suggested that the mechanism of CPD in HSP treatment mainly involved epidermal growth factor receptor (EGFR)-related targets and pathways, followed by molecular docking confirming strong binding affinity between EGFR and the bioactive components (binding energy< -4.25 kcal/mol). Real-time quantitative polymerase chain reaction and western blot showed that CPD suppressed the activation of the EGFR/extracellular signal-regulated kinase (ERK) signalling pathway (p < 0.05), which could be associated with the inhibition of complement system activation. This study provides a new perspective for understanding the pathogenesis and treatment of HSP.

摘要

过敏性紫癜(HSP)是一种由免疫球蛋白A(IgA)介导的系统性血管炎,常见于儿童,可能进展为严重并发症。复方紫癜汤(CPD)是一种经典的中药复方,对HSP的临床症状和预后有良好疗效;然而,其潜在的分子机制尚不清楚。首先,高效液相色谱分析确定了CPD中的五种生物活性成分,包括原儿茶酸、绿原酸、芒果苷、黄芩苷和醉鱼草苷,含量分别为0.011±0.02、0.577±0.33、0.150±0.05、1.132±0.23和0.369±0.23mg/g。此外,我们建立了过敏性紫癜动物模型,以评估CPD对HSP的治疗效果。CPD有效减轻了肾脏和皮肤血管炎,并减少了IgA沉积。CPD可调节Treg/Th17细胞平衡,降低促炎因子白细胞介素-6和肿瘤坏死因子-α的水平,并抑制C3和C5的表达(p<0.05)。网络药理学分析表明,CPD治疗HSP的机制主要涉及表皮生长因子受体(EGFR)相关靶点和途径,随后分子对接证实EGFR与生物活性成分之间具有很强的结合亲和力(结合能<-4.25 kcal/mol)。实时定量聚合酶链反应和蛋白质印迹表明,CPD抑制了EGFR/细胞外信号调节激酶(ERK)信号通路的激活(p<0.05),这可能与补体系统激活的抑制有关。本研究为理解HSP的发病机制和治疗提供了新的视角。

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