Recombinant expression of receptor binding domains of all eight subtypes of botulinum neurotoxin type A for generation of antitoxins with broad reactivity.

BACKGROUND

Botulinum neurotoxin type A (BoNT/A) represents a major threat to global public health because of its most potent toxicity with the longest persistence. Several camelid single-domain antibodies (or VHHs) have been reported to exhibit high neutralizing activity against the receptor binding domain (H ) of the BoNT/A subtype used to generate them. However, it remains unclear if these VHHs can neutralize effectively H of other BoNT/A subtypes. This study aimed to generate H domains of all eight BoNT/A subtypes and to screen for VHHs with broad reactivity against these domains.

METHODS

H domains of BoNT/A1-A8 were recombinantly produced in The fragment was amplified from sludge sample and cloned into pET45b vector by Gibson assembly. Expression vectors for H domains of BoNT/A2-A8 were derived from pET45b-H A1 by site-directed mutagenesis and/or in-house gene synthesis. Similarly, VHHs were synthesized and cloned into pET22b vector. Recombinant protein were purified by Ni-NTA spin columns and analyzed by SDS-PAGE. ELISA was used to confirm the antigenicity of H domains and to evaluate the reactivity of VHHs to these domains.

RESULTS

SDS-PAGE analysis and ELISA results with commercial polyclonal antibody demonstrated the H domains of all eight BoNT/A subtypes were correctly produced. ELISA results using a VHH panel indicated that, apart from ciA-C2, a well-characterized VHH specific for H of BoNT/A1, two new VHHs were found to recognize the H domains of all BoNT/A subtypes, of which VHH-A3 displayed EC values for these domains close to those of ciA-C2.

CONCLUSION

This study provided a resource to comprehensively identify antitoxins conferring broad protection against BoNT/A.