Du Qianqian, Liu Zhaoqiu, Shi Wei, Liu Xijie, Meng Qinghong, Zheng Dongyi, Yao Kaihu
Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Department of Preventive Health, Huaxin First Affiliated Hospital of Tsinghua University, Beijing, China.
Expert Rev Vaccines. 2025 Dec;24(1):206-211. doi: 10.1080/14760584.2025.2476521. Epub 2025 Mar 13.
We analyzed the impact of non-pharmacological interventions (NPIs) and PCV13 inoculation on nasopharyngeal (NP) carriage of (Sa), (Sp), (Mc), and (Hi) in healthy children under 5-years-old in Beijing, China.
NP swabs were taken from healthy children seeking routine well-child care at the pediatric preventive health clinic. NP swabs were frozen in Tryptic Soy Broth (TSB) medium and stored at -80°C, and bacterial was detected by culture.
From December 2019 to November 2021, 1939 children were enrolled, among whom 278 (14.3%) were found to carry Sa isolates, 115 (5.9%) Sp, 39 (2.0%) Mc, and 6 (0.3%) Hi. The carriage of Sa was highest in infants under 6 months, negatively correlated with Sp and Mc. The Sa carriage rate in infants below 6 months of age rose from 26.7% in pre-NPIs to 32.7% in post-NPIs early. The 13-valent pneumococcal conjugate vaccine (PCV13) uptake rose from 42.3% in December 2019 to 62.3% by October 2021.
The broad application of NPIs caused a decline in Sp and Mc carriage among children under 5-years-old, accompanied by an elevation in the Sa carriage rate among infants.
我们分析了非药物干预措施（NPIs）和13价肺炎球菌结合疫苗（PCV13）接种对中国北京5岁以下健康儿童鼻咽部（NP）携带金黄色葡萄球菌（Sa）、肺炎链球菌（Sp）、卡他莫拉菌（Mc）和流感嗜血杆菌（Hi）的影响。
从儿科预防保健门诊寻求常规儿童健康护理的健康儿童中采集鼻咽拭子。鼻咽拭子在胰蛋白胨大豆肉汤（TSB）培养基中冷冻，并储存在-80°C，通过培养检测细菌。
2019年12月至2021年11月，共纳入1939名儿童，其中278名（14.3%）携带Sa分离株，115名（5.9%）携带Sp，39名（2.0%）携带Mc，6名（0.3%）携带Hi。Sa在6个月以下婴儿中的携带率最高，与Sp和Mc呈负相关。6个月以下婴儿的Sa携带率从NPIs前的26.7%上升至NPIs早期后的32.7%。13价肺炎球菌结合疫苗（PCV13）的接种率从2019年12月的42.3%上升至2021年10月的62.3%。
NPIs的广泛应用导致5岁以下儿童Sp和Mc携带率下降，同时婴儿Sa携带率升高。
