Bartholomew J S, Handley C J, Lowther D A
Biochem J. 1985 Apr 15;227(2):429-37. doi: 10.1042/bj2270429.
The effects of mild or severe trypsin treatment of bovine articular-cartilage slices in tissue culture were studied by monitoring the incorporation of [35S]sulphate into proteoglycans. Moderate trypsin treatment caused a subsequent marked inhibition of proteoglycan biosynthesis, which was reversible with time. Analysis on Sepharose CL-2B of the proteoglycan species synthesized showed that, directly after trypsin treatment, there was a 30% increase in the synthesis of the low-Mr proteoglycan (Kav. 0.71), and the total decrease in proteoglycan biosynthesis was reflected in a decrease in the synthesis of the high-Mr proteoglycan species (Kav. 0.31). The small proteoglycan was partially characterized and shown to be a true biosynthetic product and not a breakdown product. Trypsin treatment (20 micrograms/ml per 100 mg of tissue) of cartilage slices also resulted in an increase in the glycosaminoglycan chain size of the large proteoglycan, but not of the small proteoglycan.
通过监测[35S]硫酸盐掺入蛋白聚糖的情况，研究了在组织培养中用轻度或重度胰蛋白酶处理牛关节软骨切片的效果。适度的胰蛋白酶处理随后导致蛋白聚糖生物合成受到明显抑制，这种抑制随时间是可逆的。对合成的蛋白聚糖种类进行Sepharose CL - 2B分析表明，胰蛋白酶处理后立即出现低Mr蛋白聚糖（Kav. 0.71）合成增加30%，而蛋白聚糖生物合成的总体减少反映在高Mr蛋白聚糖种类（Kav. 0.31）合成的减少上。对小蛋白聚糖进行了部分特性鉴定，结果表明它是真正的生物合成产物而非降解产物。用胰蛋白酶（每100 mg组织20微克/毫升）处理软骨切片还导致大蛋白聚糖的糖胺聚糖链大小增加，但小蛋白聚糖没有这种情况。
