Jimenez S A, McArthur W M, Bashey R I, Rosenbloom J
Arthritis Rheum. 1985 May;28(5):502-10. doi: 10.1002/art.1780280506.
We studied the effects of lymphokines from mitogen-stimulated normal human mononuclear cells on collagen production by confluent monolayer cultures of scleroderma dermal fibroblasts. We examined 10 cell lines obtained from patients with early onset, rapidly progressive disease. All cell lines exhibited increased collagen production, compared with cell lines from age- and sex-matched normal individuals. We found that supernatants from phytohemagglutinin-stimulated mononuclear cells caused greater than 45% inhibition of collagen production by the cultured scleroderma fibroblasts; the degree of inhibition was concentration-dependent. Partially purified lymphokine preparations produced 84-94% inhibition of collagen production by scleroderma cells. The lymphokine effects were shown to be selective for collagen, since the synthesis of noncollagenous proteins was not significantly affected. The results suggest that lymphokines from normal mononuclear cells can modulate the excessive collagen biosynthesis that is characteristic of scleroderma fibroblasts, and these lymphokines may have a place as possible therapeutic agents for this incurable disease.
我们研究了来自丝裂原刺激的正常人单核细胞的淋巴因子对硬皮病皮肤成纤维细胞汇合单层培养物中胶原蛋白产生的影响。我们检测了从早发型、快速进展性疾病患者中获得的10个细胞系。与年龄和性别匹配的正常个体的细胞系相比，所有细胞系的胶原蛋白产生均增加。我们发现，植物血凝素刺激的单核细胞的上清液对培养的硬皮病成纤维细胞的胶原蛋白产生有大于45%的抑制作用；抑制程度呈浓度依赖性。部分纯化的淋巴因子制剂对硬皮病细胞的胶原蛋白产生有84 - 94%的抑制作用。淋巴因子的作用显示对胶原蛋白具有选择性，因为非胶原蛋白的合成未受到显著影响。结果表明，来自正常单核细胞的淋巴因子可以调节硬皮病成纤维细胞特有的过度胶原蛋白生物合成，并且这些淋巴因子可能作为这种不治之症的潜在治疗药物。
