Chambers T J, Serafini E P
Br J Exp Pathol. 1985 Jun;66(3):309-15.
We measured the permeability of normal, adenomatous, colitic and malignant large bowel epithelial cells by immersing fragments of large bowel mucosa in radiolabelled inulin and comparing autoradiograph grain density inside and outside cells after incubation. All the carcinomas studied showed extensive uptake of inulin within 5 min, while normal, adenomatous and colitic epithelial cells completely excluded inulin for 30 min. We found no difference in the proportion of epithelial cells incorporating uridine into RNA in carcinomatous and normal mucosa, and this suggests that the increased inulin permeability of carcinoma cell membranes was not due to leakage into non-viable cells. Experiments with cytochalasin B also showed that increased pinocytosis by carcinoma cells could not account for the difference. The relative impermeability of adenomatous and colitic cells suggests that increased permeability is not caused by increased proliferation. The consistent finding of increased permeability in the plasma membranes of carcinoma cells suggests that this may be more than an epiphenomenon of malignancy. It also suggests that measurement of cell permeability may have a role in distinguishing malignant from benign epithelial neoplasms.
我们通过将大肠黏膜片段浸入放射性标记的菊粉中，并在孵育后比较细胞内外的放射自显影片颗粒密度，来测量正常、腺瘤性、结肠炎和恶性大肠上皮细胞的通透性。所有研究的癌组织在5分钟内都显示出菊粉的大量摄取，而正常、腺瘤性和结肠炎上皮细胞在30分钟内完全排除菊粉。我们发现癌组织和正常黏膜中掺入尿苷到RNA中的上皮细胞比例没有差异，这表明癌细胞膜菊粉通透性增加并非由于渗漏到无活力细胞中。用细胞松弛素B进行的实验也表明，癌细胞吞噬作用增加不能解释这种差异。腺瘤性和结肠炎细胞的相对不透性表明通透性增加不是由增殖增加引起的。癌细胞质膜通透性增加这一一致发现表明，这可能不仅仅是恶性肿瘤的一种附带现象。这也表明测量细胞通透性可能在区分恶性和良性上皮肿瘤方面具有作用。
