小鼠NIH 3T3细胞中袢利尿剂敏感的Na⁺内流与袢利尿剂敏感的K⁺净外流的偶联。

Coupling of a loop diuretic-sensitive Na+ influx with the net loop diuretic-sensitive K+ efflux in mouse NIH 3T3 cells.

作者信息

Panet R, Snyder D, Atlan H

出版信息

Biochim Biophys Acta. 1985 Jun 27;816(2):278-82. doi: 10.1016/0005-2736(85)90495-x.

Abstract

Mouse 3T3 fibroblasts have a loop diuretic sensitive Na+ transport system, responsible for more than 50% of the total Na+ influx. This transport system is dependent on the simultaneous presence of all three ions; Na+, K+, (Rb+) and Cl- in the extracellular medium. The same requirement for these three ions was also found for the loop diuretic-sensitive K+ efflux. In addition, the sensitivities of Na+ influx and Rb+ efflux for the two loop diuretics, furosemide and bumetanide were found to be similar. The similar ionic requirement and sensitivity towards loop diuretics of the two fluxes, support the hypothesis, that this loop diuretic-sensitive Na+ influx in mouse 3T3 cells, is accompanied by the net loop diuretic-sensitive K+ efflux.

摘要

小鼠3T3成纤维细胞具有一种对袢利尿剂敏感的Na⁺转运系统，该系统负责超过50%的总Na⁺内流。这种转运系统依赖于细胞外介质中同时存在所有三种离子：Na⁺、K⁺（Rb⁺）和Cl⁻。对于袢利尿剂敏感的K⁺外流，也发现了对这三种离子的相同需求。此外，发现两种袢利尿剂呋塞米和布美他尼对Na⁺内流和Rb⁺外流的敏感性相似。两种通量对袢利尿剂的相似离子需求和敏感性，支持了这样一种假设，即小鼠3T3细胞中这种对袢利尿剂敏感的Na⁺内流伴随着对袢利尿剂敏感的K⁺净外流。