Rüstow B, Kunze D
Biochim Biophys Acta. 1985 Jul 9;835(2):273-8. doi: 10.1016/0005-2760(85)90282-6.
In microsomes of rat lung, labeled diacylglycerol was synthesized from sn-[3H]glycerol 3-phosphate, which had been added, and from the endogenous free fatty acids. In these microsomes containing biosynthesized [3H]diacylglycerol as well as endogenous nonlabeled diacylglycerol, the synthesis of phosphatidylcholine was measured from added [14C]CDPcholine. The incorporation of [methyl-14C]choline and of [3H]diacylglycerol into phosphatidylcholine showed an entirely different progress in the time-course of incubation. The 14C label of phosphatidylcholine increased continuously, whereas the 3H label remained constant after 2 min up to the end of the incubation period of 20 min. From this result we concluded that the diacylglycerols, synthesized in vitro from glycerol 3-phosphate over an incubation period of 20 min, constitute a separate substrate pool for the biosynthesis of phosphatidylcholine, and are not mixed with the endogenous diacylglycerol pool.
在大鼠肺微粒体中，标记的二酰基甘油由添加的sn-[3H]甘油3-磷酸以及内源性游离脂肪酸合成。在这些含有生物合成的[3H]二酰基甘油以及内源性未标记二酰基甘油的微粒体中，通过添加的[14C]CDP胆碱来测定磷脂酰胆碱的合成。[甲基-14C]胆碱和[3H]二酰基甘油掺入磷脂酰胆碱在孵育的时间进程中显示出完全不同的进展。磷脂酰胆碱的14C标记持续增加，而3H标记在2分钟后保持恒定，直至20分钟孵育期结束。根据这一结果我们得出结论，在20分钟的孵育期内由甘油3-磷酸体外合成的二酰基甘油构成了磷脂酰胆碱生物合成的一个独立底物池，并且不与内源性二酰基甘油池混合。
