Marcenac F, Blache D
Biochim Biophys Acta. 1985 Jul 5;840(3):377-82. doi: 10.1016/0304-4165(85)90218-1.
Differential pulse voltammetry combined with electrochemically treated carbon fibre microelectrodes was used to monitor endogenous serotonin release occurring during platelet aggregation. After platelet stimulation by thrombin, an oxidation peak was recorded at +280 mV. HPLC analyses performed with fluorimetric detection have shown that this released electroactive compound was essentially serotonin. Moreover, serotonin measurements in the same samples by the technique reported here and by fluorimetry were found to be very similar (1.15 +/- 0.30 microM and 1.17 +/- 0.15 (mean +/- S.D., n = 6), respectively). Extracellular serotonin concentrations could be estimated either directly during aggregation or in supernatants obtained from stimulated or lysed platelets. Maximal serotonin concentrations have been found to be 6.93 +/- 0.37 and 3.28 +/- 0.39 nmol/10(9) platelets from rat and human, respectively. Using the reported procedure, we have observed that no serotonin was released from thrombin-stimulated platelets prepared from rats treated with reserpine. Our new technique represents a selective and performant tool for rapid determination of endogenous serotonin platelet secretion.
差分脉冲伏安法结合电化学处理的碳纤维微电极用于监测血小板聚集过程中内源性血清素的释放。用凝血酶刺激血小板后，在+280 mV处记录到一个氧化峰。采用荧光检测的高效液相色谱分析表明，这种释放出的电活性化合物主要是血清素。此外，用本文报道的技术和荧光法对同一样品中的血清素进行测量，结果非常相似（分别为1.15±0.30 microM和1.17±0.15（平均值±标准差，n = 6））。细胞外血清素浓度可以在聚集过程中直接估算，也可以在从刺激或裂解的血小板中获得的上清液中估算。已发现大鼠和人类血小板中血清素的最大浓度分别为6.93±0.37和3.28±0.39 nmol/10(9)个血小板。使用所报道的方法，我们观察到用利血平处理的大鼠制备的凝血酶刺激血小板未释放血清素。我们的新技术是一种用于快速测定内源性血清素血小板分泌的选择性和高性能工具。
