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探索卡介苗递送曼氏血吸虫抗生物素蛋白融合抗原。

Exploring BCG to deliver avidin fusion antigens from Schistosoma mansoni.

作者信息

Yamamoto Lais Sayuri, Trentini Monalisa Martins, Rodriguez Dunia, Silveira Paulo Henrique Santana, Januzzi Arthur Daniel, Carvalho Ana Carolina de Oliveira, Leite Luciana Cezar de Cerqueira, Kanno Alex Issamu

机构信息

Instituto Butantan, Laboratório de Desenvolvimento de Vacinas, São Paulo, SP, Brasil.

Universidade de São Paulo, Programa de Pós-Graduação Interunidades em Biotecnologia, São Paulo, SP, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2025 Mar 3;120:e240167. doi: 10.1590/0074-02760240167. eCollection 2025.

DOI:10.1590/0074-02760240167
PMID:40053008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11884745/
Abstract

BACKGROUND

Bacillus Calmette-Guérin (BCG) is one of the most successful vaccines in the world and evidence suggests it can be used as a bacterial vector to deliver heterologous antigens.

OBJECTIVES

We evaluated whether BCG could be biotinylated and used as a carrier of Schistosoma mansoni antigen tetraspanin-2 (TSP-2) fused with rhizavidin, an avidin analog.

METHODS

BCG was grown and biotinylated. The recombinant protein Rzv:TSP-2 was produced and purified from Escherichia coli. The biotinylation and antigen coupling was analysed by flow cytometry, enzyme-linked immunosorbent assay (ELISA) and Western blot. Vaccine immunogenicity was tested in immunised mice by the assessment of lung and splenic T cells.

FINDINGS

BCG can be biotinylated, which in turn, can be coupled with Rzv:TSP-2. After a series of optimisations which involved molarity of the biotin, ratio of BCG:reagent and the concentration of Rzv:TSP-2 used, almost 50% of the bacteria were biotinylated and 35% coupled with antigen. Although a clear adjuvant effect of BCG was observed, evaluation of immune response in immunised mice demonstrated an overall low immunogenicity of the BCG-Rzv:TSP-2.

MAIN CONCLUSION

These results demonstrated the use of BCG as a carrier of avidin-tagged antigens. Further optimisations are needed in order to strengthen the stability of tagged proteins in order to produce antigen-specific immune responses.

摘要

背景

卡介苗（BCG）是世界上最成功的疫苗之一，有证据表明它可作为一种细菌载体来递送异源抗原。

目的

我们评估了卡介苗是否可以生物素化，并用作与抗生物素蛋白类似物根抗生物素蛋白融合的曼氏血吸虫抗原四跨膜蛋白-2（TSP-2）的载体。

方法

培养并生物素化卡介苗。从大肠杆菌中生产并纯化重组蛋白Rzv:TSP-2。通过流式细胞术、酶联免疫吸附测定（ELISA）和蛋白质印迹法分析生物素化和抗原偶联情况。通过评估肺和脾中的T细胞，在免疫小鼠中测试疫苗的免疫原性。

研究结果

卡介苗可以生物素化，进而可与Rzv:TSP-2偶联。经过一系列优化，包括生物素的摩尔浓度、卡介苗与试剂的比例以及所用Rzv:TSP-2的浓度，几乎50%的细菌被生物素化，35%与抗原偶联。尽管观察到卡介苗有明显的佐剂作用，但对免疫小鼠免疫反应的评估表明，卡介苗-Rzv:TSP-2的总体免疫原性较低。

主要结论

这些结果证明了卡介苗可作为抗生物素蛋白标记抗原的载体。为了增强标记蛋白的稳定性以产生抗原特异性免疫反应，还需要进一步优化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/983cae563bb6/1678-8060-mioc-120-e240167-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/d926f60988c4/1678-8060-mioc-120-e240167-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/fd16904db792/1678-8060-mioc-120-e240167-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/45ba7cee3f19/1678-8060-mioc-120-e240167-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/3d334a1046c4/1678-8060-mioc-120-e240167-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/5d3750b96b62/1678-8060-mioc-120-e240167-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/983cae563bb6/1678-8060-mioc-120-e240167-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/d926f60988c4/1678-8060-mioc-120-e240167-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/fd16904db792/1678-8060-mioc-120-e240167-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/45ba7cee3f19/1678-8060-mioc-120-e240167-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/3d334a1046c4/1678-8060-mioc-120-e240167-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/5d3750b96b62/1678-8060-mioc-120-e240167-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/537d/11884745/983cae563bb6/1678-8060-mioc-120-e240167-gf6.jpg

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本文引用的文献

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2
Vaccine development: Current trends and technologies.疫苗研发：当前趋势与技术。
Life Sci. 2024 Jan 1;336:122331. doi: 10.1016/j.lfs.2023.122331. Epub 2023 Dec 7.
3
Parenteral BCG vaccine induces lung-resident memory macrophages and trained immunity via the gut-lung axis.BCG 疫苗通过肠肺轴诱导肺驻留记忆巨噬细胞和训练性免疫。
Nat Immunol. 2022 Dec;23(12):1687-1702. doi: 10.1038/s41590-022-01354-4. Epub 2022 Dec 1.
4
Robust Immune Response Induced by TSP-2 Antigen Coupled to Bacterial Outer Membrane Vesicles.TSP-2 抗原偶联细菌外膜囊泡诱导的强大免疫应答。
Int J Nanomedicine. 2021 Oct 22;16:7153-7168. doi: 10.2147/IJN.S315786. eCollection 2021.
5
Thirty years of recombinant BCG: new trends for a centenary vaccine.重组卡介苗三十年：百年疫苗的新趋势
Expert Rev Vaccines. 2021 Aug;20(8):1001-1011. doi: 10.1080/14760584.2021.1951243. Epub 2021 Jul 13.
6
Optimization of Expression and Purification of Schistosoma mansoni Antigens in Fusion with Rhizavidin.与亲和素融合表达和纯化曼氏血吸虫抗原的优化。
Mol Biotechnol. 2021 Nov;63(11):983-991. doi: 10.1007/s12033-021-00355-2. Epub 2021 Jun 24.
7
A guide to vaccinology: from basic principles to new developments.疫苗学指南：从基本原则到新进展。
Nat Rev Immunol. 2021 Feb;21(2):83-100. doi: 10.1038/s41577-020-00479-7. Epub 2020 Dec 22.
8
BCG as a Vector for Novel Recombinant Vaccines against Infectious Diseases and Cancers.卡介苗作为针对传染病和癌症的新型重组疫苗载体
Vaccines (Basel). 2020 Dec 4;8(4):736. doi: 10.3390/vaccines8040736.
9
Prevention of tuberculosis in macaques after intravenous BCG immunization.静脉内 BCG 免疫后猕猴结核病的预防。
Nature. 2020 Jan;577(7788):95-102. doi: 10.1038/s41586-019-1817-8. Epub 2020 Jan 1.
10
Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology.寨卡病毒ΔNS1在大肠杆菌中的优化及放大生产：响应面法的应用
AMB Express. 2019 Dec 31;10(1):1. doi: 10.1186/s13568-019-0926-y.