Mueller H J
Biomaterials. 1985 May;6(3):146-9. doi: 10.1016/0142-9612(85)90001-8.
Employing equilibrium dialysis, the binding abilities of Cu, Al, Co and Cr ions from corroded Cu-Al and Co-Cr dental casting alloys towards human saliva and two of its gel chromatographic fractions were determined. Results indicate that both Cu and Co bind to human saliva i.e. 0.045 and 0.027 mg/mg protein, respectively. Besides possessing the largest binding ability, Cu also possessed the largest binding capacity. The saturation of Cu binding was not reached up to the limit of 0.35 mg protein/ml employed in the tests, while Co reached full saturation at about 0.2 mg protein/ml. Chromium showed absolutely no binding to human saliva while Al ions did not pass through the dialysis membranes. Compared to the binding with solutions that were synthetically made up to contain added salivary-type proteins, it is shown that the binding to human saliva is about 1 order of magnitude larger, at least for Cu ions.
采用平衡透析法，测定了腐蚀后的铜 - 铝和钴 - 铬牙科铸造合金中的铜、铝、钴和铬离子对人唾液及其两个凝胶色谱馏分的结合能力。结果表明，铜和钴均与人唾液结合，即分别为0.045和0.027毫克/毫克蛋白质。除了具有最大的结合能力外，铜还具有最大的结合容量。在测试所用的0.35毫克蛋白质/毫升的极限浓度下，铜结合未达到饱和，而钴在约0.2毫克蛋白质/毫升时达到完全饱和。铬与人唾液完全不结合，而铝离子未透过透析膜。与与合成添加唾液型蛋白质的溶液的结合相比，结果表明，至少对于铜离子而言，与人唾液的结合大约大一个数量级。
