聚甲基丙烯酸2-羟乙酯 - 胶原蛋白复合材料，其在体外促进肌肉细胞分化。

Poly(2-hydroxyethyl methacrylate)--collagen composites which promote muscle cell differentiation in vitro.

作者信息

Stol M, Tolar M, Adam M

出版信息

Biomaterials. 1985 May;6(3):193-7. doi: 10.1016/0142-9612(85)90009-2.

Abstract

A new simple method has been developed which allows the mixing of poly(2-hydroxyethyl methacrylate)--polyHEMA--and fibrillar collagen in any desired ratio. PolyHEMA alone was shown to be an unsuitable cultivation substrate for primary cultures of chicken embryonic skeletal muscle cells. Composites containing polyHEMA and 50% (w/w) or more collagen supported myogenesis. Such layers, firmly adhered to the bottom of plastic Petri dishes, were mechanically stable and biologically active, thus favourably combining properties of both the original materials. It is suggested that polyHEMA-collagen composite layers may be used for cultivation of differentiating cells in vitro.

摘要

已开发出一种新的简单方法，该方法能够以任何所需比例将聚甲基丙烯酸羟乙酯（poly(2-hydroxyethyl methacrylate)，简称polyHEMA）与纤维状胶原蛋白混合。单独的聚甲基丙烯酸羟乙酯被证明是鸡胚骨骼肌细胞原代培养不合适的培养基质。含有聚甲基丙烯酸羟乙酯和50%（w/w）或更多胶原蛋白的复合材料支持肌细胞生成。这种牢固附着在塑料培养皿底部的层在机械上是稳定的且具有生物活性，因此有利地结合了两种原始材料的特性。有人提出聚甲基丙烯酸羟乙酯 - 胶原蛋白复合层可用于体外分化细胞的培养。