Li Xiaosen, Wang Zhenpeng, Man Xiaxia, Dai Xiangpeng, Zhou Qi, Zhang Songling
Department of Gynecologic Oncology, Gynecology and Obstetrics Centre, The First Hospital of Jilin University, Changchun, China; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Department of Gynecologic Oncology, Gynecology and Obstetrics Centre, The First Hospital of Jilin University, Changchun, China.
Gynecol Oncol. 2025 Apr;195:34-44. doi: 10.1016/j.ygyno.2025.02.022. Epub 2025 Mar 6.
Epithelial ovarian carcinoma (EOC), the most lethal gynecologic cancer, is often diagnosed at advanced stages, which urge us to explore the novel therapeutic strategies. Mouse models have played a crucial role in elucidating the molecular mechanisms for the development ovarian cancer and its therapeutic strategies. However, there are still various challenges in modeling the genetic drivers of ovarian cancer in animal models. Here, we provided an overview of the research advances for the molecular mechanisms underlying EOC development, therapeutic strategies, the CRISPR genome editing technology and its generated EOC models. The review also comprehensively discussed the advantages and obstacles of CRISPR in generating EOC mouse models and the promising therapeutic approach by correcting the oncogenes of EOC through in vivo delivery of gene-edited components. The development of more precise animal models, along with a deeper understanding of EOC molecular mechanisms, will dramatically benefit the investigation and treatment of EOC.
上皮性卵巢癌（EOC）是最致命的妇科癌症，通常在晚期才被诊断出来，这促使我们探索新的治疗策略。小鼠模型在阐明卵巢癌发生发展的分子机制及其治疗策略方面发挥了关键作用。然而，在动物模型中模拟卵巢癌的基因驱动因素仍存在各种挑战。在此，我们概述了EOC发生发展的分子机制、治疗策略、CRISPR基因组编辑技术及其构建的EOC模型的研究进展。本综述还全面讨论了CRISPR在构建EOC小鼠模型中的优势和障碍，以及通过体内递送基因编辑成分纠正EOC癌基因的有前景的治疗方法。开发更精确的动物模型，以及更深入地了解EOC分子机制，将极大地有利于EOC的研究和治疗。
