Leitzke Amanda Fonseca, Bueno Danielle Tapia, Jansen-Alves Cristina, Trindade Tamara Mendes Leite Silva, Pedra Nathalia Stark, Santana Luiza Ribeiro, Stefanello Francieli Moro, da Rosa Zavareze Elessandra, Borsuk Sibele, Carreño Neftali Lenin Villarreal, de Pereira Claudio Martin Pereira
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Eliseu Maciel St., s/n, Pelotas, RS 96160-000, Brazil; Postgraduate Program in Biotechnology, Federal University of Pelotas, Eliseu Maciel St., s/n, Pelotas, RS 96160-000, Brazil.
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Eliseu Maciel St., s/n, Pelotas, RS 96160-000, Brazil; Postgraduate Program in Materials Science and Engineering, Technology Development Center, Federal University of Pelotas, 96010-000 Pelotas, Rio Grande do Sul, Brazil.
Int J Biol Macromol. 2025 May;306(Pt 4):141788. doi: 10.1016/j.ijbiomac.2025.141788. Epub 2025 Mar 5.
Ultrafine fibers are gaining attention for their unique properties and wide-ranging applications. Among production techniques, electrospinning stands out for its cost-effectiveness, simplicity, and control over fiber structure and diameter. This study reports for the first time the development of ultrafine fibers using zein, a renewable protein, with the addition of fucoidan, a bioactive polysaccharide from brown algae, through electrospinning. Polymeric solutions of 30 % zein and varying concentrations of fucoidan (0 %, 25 %, 30 %, 35 %) were produced, with viscosity and electrical conductivity analyzed. Solutions with 25 % and 30 % fucoidan showed higher viscosity, while conductivity remained relatively stable. The hydrophilic nature of the solutions, indicated by contact angle analysis, favors cell adhesion and proliferation. Fourier transform infrared and X-ray diffraction analyses confirmed the material characteristics and amorphous nature of the fibers. Ultrafine fibers distribution averaged 540 ± 130 nm, with those incorporating 25 % and 30 % fucoidan forming ribbon shapes and smaller diameters (234 ± 54 nm and 276 ± 54 nm), which suggests improved fiber structure, potentially enhancing biological activity. Cytotoxicity tests on mouse fibroblast cells showed no toxic effects after 24 and 48 h, supporting the feasibility of further biological studies, including the potential use of these fibers as skin healing adhesives.
超细纤维因其独特性能和广泛应用而备受关注。在生产技术中，静电纺丝因其成本效益高、操作简单以及能够控制纤维结构和直径而脱颖而出。本研究首次报道了通过静电纺丝利用玉米醇溶蛋白（一种可再生蛋白质）并添加岩藻依聚糖（一种来自褐藻的生物活性多糖）来制备超细纤维。制备了30%玉米醇溶蛋白和不同浓度岩藻依聚糖（0%、25%、30%、35%）的聚合物溶液，并对其粘度和电导率进行了分析。含有25%和30%岩藻依聚糖的溶液显示出较高的粘度，而电导率保持相对稳定。通过接触角分析表明，溶液的亲水性有利于细胞粘附和增殖。傅里叶变换红外光谱和X射线衍射分析证实了纤维的材料特性和无定形性质。超细纤维的平均直径为540±130nm，其中含有25%和30%岩藻依聚糖的纤维形成带状且直径较小（分别为234±54nm和276±54nm），这表明纤维结构得到改善，可能会增强生物活性。对小鼠成纤维细胞的细胞毒性测试表明，在24小时和48小时后均无毒性作用，这支持了进一步开展生物学研究的可行性，包括这些纤维作为皮肤愈合粘合剂的潜在用途。
