Bal Altuntaş Derya, Sanko Vildan, Kuralay Filiz
Department of Bioengineering, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53100, Turkey.
Department of Chemistry, Faculty of Science, Hacettepe University, Ankara 06800, Turkey; Middle East Technical University, Micro-Electro-Mechanical Systems (MEMS) Center, Ankara 06530, Turkey.
J Pharm Biomed Anal. 2025 Aug 1;260:116780. doi: 10.1016/j.jpba.2025.116780. Epub 2025 Feb 27.
The development of functional interfaces for healthcare applications is in great demand to perform sensitive and reliable testing. For this purpose, in the present study, the use of transition metal dichalcogenides (TMDCs) combined with carbon nanotubes modified pencil graphite electrode (PGE) was demonstrated for fish sperm double-stranded deoxyribonucleic acid (fsDNA) detection. The advanced system consisted of molybdenum disulfide (MoS), tungsten disulfide (WS), and multi-walled carbon nanotubes (MWCNTs), which exhibited superior and fascinating electrochemical properties on PGE as a result of synergetic effect occurred between the materials. The MoS-WS-MWCNTs incorporated PGE (MoS-WS-MWCNTs/E) was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD) analysis, and electrochemical techniques. The elemental composition of the sensing platform was monitored with energy-dispersive X-ray spectroscopy (EDS). Specific parameters, including the amount of components in the hybrid material were optimized. MoS-WS-MWCNTs/E presented a good linear range for fsDNA between 0.1 and 50mgL with a low detection limit of 0.05 mg L by using the differential pulse voltammetry (DPV) technique. The detection performance of the resulting electrode was compared with the responses of MoS-MWCNTs/E and WS-MWCNTs/E as well. This sensing platform establishes the use of dichalcogenides-carbon nanotubes-based material for facile, sensitive, and reproducible sensing applications.
为了进行灵敏且可靠的测试，医疗保健应用中对功能性接口的开发有很大需求。为此，在本研究中，展示了将过渡金属二硫属化物（TMDCs）与碳纳米管修饰的铅笔石墨电极（PGE）结合用于鱼精子双链脱氧核糖核酸（fsDNA）检测。先进的系统由二硫化钼（MoS）、二硫化钨（WS）和多壁碳纳米管（MWCNTs）组成，由于材料之间产生协同效应，它们在PGE上表现出优异且迷人的电化学性能。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、X射线衍射（XRD）分析和电化学技术对MoS-WS-MWCNTs修饰的PGE（MoS-WS-MWCNTs/E）进行了表征。用能量色散X射线光谱（EDS）监测传感平台的元素组成。对包括混合材料中各组分含量在内的特定参数进行了优化。通过差分脉冲伏安法（DPV）技术，MoS-WS-MWCNTs/E对fsDNA的线性范围良好（0.1至50mg/L），检测限低至0.05mg/L。还将所得电极的检测性能与MoS-MWCNTs/E和WS-MWCNTs/E的响应进行了比较。该传感平台确立了基于二硫属化物-碳纳米管材料在简便、灵敏和可重复传感应用中的用途。
