离子对沙眼衣原体黏附及被HeLa细胞内化的影响。
The effect of ions on the adhesion and internalization of Chlamydia trachomatis by HeLa cells.
作者信息
Sneddon J M, Wenman W M
出版信息
Can J Microbiol. 1985 Apr;31(4):371-4. doi: 10.1139/m85-071.
The adhesion and internalization of Chlamydia trachomatis by HeLa cells was unaffected by removal of K+, Mg2+, or glucose from the incubation medium, slightly reduced by removal of Na+, and significantly reduced by omission of Ca2+, Sr2+, Mg2+, and Mn2+ could replace Ca2+ in the adhesion but only Sr2+ supported internalization, and La3+, Co2+, Fe3+, Ba2+, and Zn2+ all reduced internalization more than adhesion. During initial infection there was no measurable difference in the uptake or release of 45Ca2+ or 86Rb+ between infected and noninfected HeLa monolayers. Infection was not prevented by pretreatment of the monolayers with the calcium channel blockers, verapamil, D600, and nitrendipine, or the calmodulin inhibitors, TMB-8 or trifluperazine. The results suggest that divalent cations are not essential for chlamydial infection but that the process of internalization is facilitated by the presence of cations, particularly Na+ and Ca2+.
沙眼衣原体与HeLa细胞的黏附和内化不受孵育培养基中K⁺、Mg²⁺或葡萄糖去除的影响，Na⁺去除使其略有降低，而Ca²⁺、Sr²⁺、Mg²⁺和Mn²⁺的缺失则使其显著降低，Mg²⁺和Mn²⁺可在黏附中替代Ca²⁺，但只有Sr²⁺支持内化，并且La³⁺、Co²⁺、Fe³⁺、Ba²⁺和Zn²⁺对内化的降低作用均大于对黏附的降低作用。在初始感染期间，感染和未感染的HeLa单层细胞之间45Ca²⁺或86Rb⁺的摄取或释放没有可测量的差异。单层细胞用钙通道阻滞剂维拉帕米、D600和尼群地平或钙调蛋白抑制剂TMB - 8或三氟拉嗪预处理并不能阻止感染。结果表明二价阳离子对于衣原体感染并非必不可少，但阳离子尤其是Na⁺和Ca²⁺的存在促进了内化过程。
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