Kasrineh Farzaneh Abasnezhad, Esmaeili Ozra Sadat, Tavakoli Tayyebeh, Khalili Parvin, Rajabi Zohreh, Vatankhah Hajar, Hajizadeh Mohammad Reza, Mahmoodi Mehdi, Hakimi Hamid, Jalali Zahra
Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Physiology, School of Medicine, Rafsanjan University of Medical Science, Rafsanjan, Iran.
BMC Res Notes. 2025 Mar 8;18(1):102. doi: 10.1186/s13104-025-07118-1.
Irisin is a myokine mainly produced by skeletal muscle that impacts the body's systemic metabolism. It is connected to aging, telomere length, and oxidative stress markers in human adults and in vitro. The serum irisin concentration increases during pregnancy and has been linked to some birth outcomes like macrosomia. On the other hand, its inverse relation with the chance of pregnancy disorders like preeclampsia and gestational diabetes suggests a protective role for this myokine in pregnancy. It is suggested that irisin may exert its impact on pregnancy by affecting the placenta, which has not been studied yet. Here, we questioned whether prenatal serum irisin is related to placental markers, including telomere length and antioxidant activity. Research has shown that the status of these markers at birth can predict the predisposition to some chronic diseases later in life. We included 80 pregnant mothers (17-41 years old) and newborn dyads randomly selected from the enrolled participants of the Rafsanjan Birth Cohort Study (one of the five district areas of the PERSIAN birth cohort studies), who delivered at the Nik-Nafs Maternity Hospital in Rafsanjan in 2022. Irisin levels were measured in the mother's blood serum in pregnancy using ELISA. The relative telomere length and the GPX and SOD enzyme activities were measured in the term placenta using real-time PCR and colorimetric assays, respectively. We found an inverse relationship between the serum irisin levels during pregnancy and relative telomere length in the term placenta. Irisin level was not significantly associated with the activity of SOD and GPX enzymes. Therefore, our data does not support the protective role of prenatal irisin on the placental telomere shortening and oxidative stress. Future studies are warranted to assess more placental markers in relation to pregnancy irisin levels.
鸢尾素是一种主要由骨骼肌产生的肌动蛋白，会影响人体的全身代谢。在成年人和体外实验中，它与衰老、端粒长度和氧化应激标志物相关。孕期血清鸢尾素浓度会升高，并且与一些出生结局有关，如巨大儿。另一方面，它与子痫前期和妊娠期糖尿病等妊娠疾病发生几率呈负相关，这表明这种肌动蛋白在孕期具有保护作用。有研究表明，鸢尾素可能通过影响胎盘发挥其对妊娠的作用，但尚未对此进行研究。在此，我们探讨产前血清鸢尾素是否与胎盘标志物有关，包括端粒长度和抗氧化活性。研究表明，这些标志物在出生时的状态可以预测日后患某些慢性疾病的易感性。我们纳入了80对母婴（母亲年龄17 - 41岁），他们是从拉夫桑詹出生队列研究（波斯出生队列研究五个地区之一）的登记参与者中随机选取的，于2022年在拉夫桑詹的尼克 - 纳夫斯妇产医院分娩。采用酶联免疫吸附测定法检测孕期母亲血清中的鸢尾素水平。分别采用实时荧光定量聚合酶链反应和比色法检测足月胎盘的相对端粒长度以及谷胱甘肽过氧化物酶（GPX）和超氧化物歧化酶（SOD）的活性。我们发现孕期血清鸢尾素水平与足月胎盘的相对端粒长度呈负相关。鸢尾素水平与SOD和GPX酶的活性无显著相关性。因此，我们的数据不支持产前鸢尾素对胎盘端粒缩短和氧化应激具有保护作用的观点。未来有必要开展更多研究，以评估与孕期鸢尾素水平相关的更多胎盘标志物。
