Fan Qing, Liu Lian, Wang Liwen, Yang Ruoxin, Liu Xueting, Dong Yan, Zeng Xiaoqun, Liu Xinanbei, Du Qiwei, Wu Zhen, Pan Daodong
State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China; College of Food Science and Engineering, Ningbo University, Ningbo 315800, China; Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo 315800, China; College of Resources and Environment, Baoshan University, Baoshan 67800, China.
State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China; College of Food Science and Engineering, Ningbo University, Ningbo 315800, China; Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo 315800, China.
Int J Biol Macromol. 2025 May;307(Pt 1):141863. doi: 10.1016/j.ijbiomac.2025.141863. Epub 2025 Mar 7.
Nanocoating represents an effective strategy for creating a protective barrier on probiotic surfaces, preventing them from damage. Here, we developed HAQ microcapsules comprising Limosilactobacillus fermentum RC4, which were nanocoated with hyaluronic acid and quinoa protein. We characterized the stability and safety, and investigated the intermolecular forces and transcriptome to elucidate the mechanisms underlying the nanocoating. The encapsulation efficiency, survival rates following freeze drying, simulated oro-gastrointestinal conditions, and storage at 4 °C for 56 d were 10.32 %, 12.74 %, 7.56 %, and 14.56 % higher, respectively, than those of LF RC4 alone. The HAQ microcapsules demonstrated adhesion to Caco-2 cells and safely promoted proliferation in RAW 264.7 cells. Electrostatic and hydrophobic interactions emerged as the primary forces within the HAQ microcapsules, facilitating structural rearrangements of wall materials, promoting the ordered aggregation of quinoa protein, and enhancing the stability of microcapsules. Transcriptome analysis revealed that HAQ upregulated argF and carB involved in lysine and glutamic acid biosynthesis, while downregulating mraY and murG associated with carbohydrate biosynthesis. It is postulated that these regulatory effects may enhance bacterial metabolism and proliferation, thereby facilitating the exertion of functional properties such as adhesion. Our findings offer valuable insights into the development of highly active and stable probiotic freeze-dried powders.
纳米涂层是一种在益生菌表面形成保护屏障、防止其受损的有效策略。在此，我们开发了包含发酵乳杆菌RC4的HAQ微胶囊，并用透明质酸和藜麦蛋白对其进行纳米涂层处理。我们对其稳定性和安全性进行了表征，并研究了分子间作用力和转录组，以阐明纳米涂层的潜在机制。与单独的LF RC4相比，其包封率、冻干后、模拟口腔-胃肠道条件下以及在4℃储存56天后的存活率分别提高了10.32%、12.74%、7.56%和14.56%。HAQ微胶囊表现出对Caco-2细胞的粘附性，并能安全地促进RAW 264.7细胞的增殖。静电和疏水相互作用是HAQ微胶囊内的主要作用力，促进了壁材的结构重排，促进了藜麦蛋白的有序聚集，并增强了微胶囊的稳定性。转录组分析表明，HAQ上调了参与赖氨酸和谷氨酸生物合成的argF和carB，同时下调了与碳水化合物生物合成相关的mraY和murG。据推测，这些调节作用可能会增强细菌的代谢和增殖，从而促进诸如粘附等功能特性的发挥。我们的研究结果为开发高活性和稳定的益生菌冻干粉提供了有价值的见解。
