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使用共聚焦激光扫描显微镜对四种修复材料微渗漏的比较评估:一项体外研究。

Comparative evaluation of microleakage of four restorative materials using confocal laser scanning microscopy: an in vitro study.

作者信息

Kini Annapoorna, G Kavana M, N Shubhashini, Shetty Nithin, Venkataiah Venkata Suresh, Fareed Mohammad, Karobari Mohmed Isaqali

机构信息

Rajarajeswari Dental College & Hospital, No.14, Ramohalli Cross, Kumbalgodu, Bengaluru, 560 074, India.

Ramaiah University of Applied Sciences, Bengaluru, 5600054, India.

出版信息

Ir J Med Sci. 2025 Jun;194(3):1105-1111. doi: 10.1007/s11845-025-03927-2. Epub 2025 Mar 10.

Abstract

OBJECTIVE

Understanding the sealing ability of restorative materials to prevent microleakage and its consequent complications-such as secondary caries, postoperative sensitivity, and restoration failure-is imperative in clinical dentistry. This study aims to compare the microleakage performance of four restorative materials-bioactive composite resin (Activa Pronto), alkasite restorative (Cention N), compomer (Dyract Flow), and glass ionomer cement (Type 2 GIC)-in Class V cavities of extracted premolar teeth.

MATERIALS AND METHODS

Forty non-carious, intact premolar teeth were selected and prepared with standardized Class V cavities. The teeth were randomly assigned to one of four groups (n = 10) and restored with the respective materials. The restorations underwent thermocycling, followed by microleakage testing using rhodamine dye. Confocal laser scanning microscopy (CLSM), a technique that provides superior depth resolution and three-dimensional visualization of microleakage, was used to assess dye penetration at the tooth-restoration interface. Microleakage was then scored using a standardized grading system.

RESULTS

Significant differences in microleakage were observed among the groups (χ = 45.69; p < 0.001). Group 1 (bioactive composite resin) demonstrated the lowest microleakage, with predominantly Grade 0 and Grade 1 scores, while Group 4 (GIC) exhibited the highest microleakage, characterized mainly by Grade 3 scores. Bioactive composite resin exhibited significantly lower microleakage than alkasite, compomer, and GIC (p < 0.05). No significant difference was observed between alkasite and compomer.

CONCLUSIONS

Bioactive composite resin (Activa Pronto) provided the most effective seal against microleakage, followed by alkasite and compomer, with glass ionomer cement showing the least effective sealing properties.

摘要

目的

了解修复材料防止微渗漏的封闭能力及其随之而来的并发症,如继发龋、术后敏感和修复失败,在临床牙科中至关重要。本研究旨在比较四种修复材料——生物活性复合树脂(Activa Pronto)、碱式硅铝酸盐修复材料(Cention N)、复合体(Dyract Flow)和玻璃离子水门汀(2型玻璃离子水门汀)——在拔除的前磨牙Ⅴ类洞中的微渗漏性能。

材料与方法

选取40颗无龋、完整的前磨牙,制备标准化的Ⅴ类洞。将牙齿随机分为四组(n = 10),并用相应材料进行修复。修复体进行热循环,然后使用罗丹明染料进行微渗漏测试。共聚焦激光扫描显微镜(CLSM)是一种提供卓越深度分辨率和微渗漏三维可视化的技术,用于评估牙齿-修复体界面处的染料渗透情况。然后使用标准化分级系统对微渗漏进行评分。

结果

各组间微渗漏存在显著差异(χ = 45.69;p < 0.001)。第1组(生物活性复合树脂)微渗漏最低,主要为0级和1级评分,而第4组(玻璃离子水门汀)微渗漏最高,主要为3级评分。生物活性复合树脂的微渗漏显著低于碱式硅铝酸盐、复合体和玻璃离子水门汀(p < 0.05)。碱式硅铝酸盐和复合体之间未观察到显著差异。

结论

生物活性复合树脂(Activa Pronto)对微渗漏提供了最有效的封闭,其次是碱式硅铝酸盐和复合体,玻璃离子水门汀的封闭性能最差。

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