Leamdum Chonticha, Phruksaphithak Nantharat, Niyasom Chaisit, Birkeland Nils Kare, Mamimin Chonticha, O-Thong Sompong
International College, Thaksin University, Songkhla, 90000, Thailand; Center of Excellence for Agricultural Innovation and Bioproducts of Thaksin University, Phatthalung, 93210, Thailand; Biofuel and Biocatalysis Innovation Research Unit, Nakhonsawan Campus, Mahidol University, Nakhonsawan, 60130, Thailand.
Center of Excellence for Agricultural Innovation and Bioproducts of Thaksin University, Phatthalung, 93210, Thailand; Department of Physical Science, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung, 93210, Thailand.
J Environ Manage. 2025 Apr;379:124831. doi: 10.1016/j.jenvman.2025.124831. Epub 2025 Mar 9.
This study demonstrated the effectiveness of enriched anoxic methanotrophic consortium augmentation in reducing methane (CH) emissions from rice cultivation while improving soil fertility and rice productivity. The enriched consortium from cattle farm effluent, dominated by Acinetobacter (65.5%) and containing both types I (Methylosarcina, Methylomagnum, and Methyloversatilis) and II (Methylocystis) methanotrophs, exhibited high methane oxidation rates (V 45.70 ± 13.71 μmol-CH⋅g⋅h and K 16.50 ± 4.95 μM). The optimal inoculum size for soil application was 0.2 L⋅m (OD = 0.5), resulting in a CH reduction efficiency of 74.30 ± 3.56%. In rice pot experiments, the anoxic methanotrophic consortium with an inoculum size of 0.2 L⋅m reduced methane emissions by 79.32 ± 3.96% without fertilizer and 29.22 ± 1.45% and 46.81 ± 1.87% when combined with organic and chemical fertilizers, respectively. The field-scale evaluation revealed that combined soil and irrigation water application with anoxic methanotrophic consortium augmentation was the most effective, reducing seasonal methane emissions from 32.8 ± 4.2 to 9.3 ± 1.5 g-CH·m and methane flux from 15.2 ± 2.1 to 4.3 ± 0.8 mg-CH·m·h, representing a 71.7 ± 0.4% reduction. This method also increased plant height (6.5%) and tiller number (26.4%). The combined application method also resulted in the highest soil nutrient levels (96.1 mg-N·kg soil, 21.8 mg-P·kg soil, and 133.4 mg-K·kg soil) and increased rice yield by 14.7% (975 g⋅m). These findings demonstrate that anoxic methanotrophic consortium augmentation is a sustainable approach to mitigate methane emissions and improve rice productivity, emphasizing the importance of integrating this strategy into rice cultivation practices in rainfed lowland areas.
本研究证明了强化缺氧甲烷营养菌联合体在减少水稻种植甲烷（CH）排放方面的有效性，同时提高了土壤肥力和水稻产量。从奶牛场废水中富集得到的联合体，以不动杆菌为主（65.5%），包含I型（甲基肉球菌、甲基巨球菌和甲基嗜温菌）和II型（甲基孢囊菌）甲烷营养菌，表现出较高的甲烷氧化速率（V 45.70±13.71 μmol-CH⋅g⋅h和K 16.50±4.95 μM）。土壤施用的最佳接种量为0.2 L⋅m（OD = 0.5），甲烷减排效率为74.30±3.56%。在水稻盆栽试验中，接种量为0.2 L⋅m的缺氧甲烷营养菌联合体在不施肥时可减少79.32±3.96%的甲烷排放，与有机肥和化肥混合施用时分别减少29.22±1.45%和46.81±1.87%。田间规模评估表明，将缺氧甲烷营养菌联合体强化与土壤和灌溉水联合施用最为有效，可将季节性甲烷排放量从32.8±4.2降至9.3±1.5 g-CH·m，甲烷通量从15.2±2.1降至4.3±0.8 mg-CH·m·h，降幅达71.
