Çolak Naki, Savcı Ahmet, Turan Nevin, Buldurun Kenan
Department of Chemistry, Faculty of Arts and Sciences, Hitit University, Çorum, Turkey.
Department of Molecular Biology and Genetics, Faculty of Art and Science, Mus Alparslan University, Mus, Turkey.
J Biochem Mol Toxicol. 2025 Mar;39(3):e70215. doi: 10.1002/jbt.70215.
This research focused on synthesizing a Schiff base (L) and its Co(II) and Pd(II) complexes under appropriate conditions to contribute to enhancing antioxidant activity. The ligand was synthesized by reacting methyl 2-amino-4-ethyl-5-methylthiophene-3-carboxylate with 5-bromo-2-hydroxybenzaldehyde. Various analytical techniques were employed to characterize the ligand and its resulting complexes, including microanalysis, H and C NMR spectroscopy, FTIR, electronic spectra, mass spectrometry, magnetic susceptibility, molar conductance, and thermo-gravimetric analysis. Electronic spectra and magnetic moment values were used to confirm the geometric structures of the synthesized compounds. Thermo-gravimetric analyses (TGA-DTA) were conducted to evaluate the thermal stability of the complexes. The Pd(II) complex was found to have a square planar structure, while a tetrahedral structure was suggested for the Co(II) complex. The results from the elemental analysis were consistent with both the ligand and metal complexes, showing good agreement between calculated and experimental values. The in vitro antioxidant activities of the newly synthesized compounds were examined using different antioxidant methods. Experiment results exhibited that the Pd(II) complex had better activities than the Schiff base and Co(II) complex. According to ABTS experimental results, Pd(II) complex (IC50:1.25) showed similar activity to the standards. In contrast, CUPRAC results revealed that Pd(II) complex (7.55 µg TE/mL) showed better activity than the standards. The results indicate that Pd(II) complex has strong potential as effective antioxidants, making them valuable for food and medicine applications.
本研究着重于在适当条件下合成一种席夫碱（L）及其钴（II）和钯（II）配合物，以助力增强抗氧化活性。该配体通过使2-氨基-4-乙基-5-甲基噻吩-3-羧酸甲酯与5-溴-2-羟基苯甲醛反应合成。采用了多种分析技术对配体及其生成的配合物进行表征，包括微量分析、氢和碳核磁共振光谱、傅里叶变换红外光谱、电子光谱、质谱、磁化率、摩尔电导率以及热重分析。利用电子光谱和磁矩值来确认合成化合物的几何结构。进行热重分析（TGA-DTA）以评估配合物的热稳定性。发现钯（II）配合物具有平面正方形结构，而钴（II）配合物则推测为四面体结构。元素分析结果与配体和金属配合物均相符，计算值与实验值显示出良好的一致性。使用不同的抗氧化方法对新合成化合物的体外抗氧化活性进行了检测。实验结果表明，钯（II）配合物的活性优于席夫碱和钴（II）配合物。根据ABTS实验结果，钯（II）配合物（IC50：1.25）显示出与标准物相似的活性。相比之下，CUPRAC结果表明，钯（II）配合物（7.55μg TE/mL）的活性优于标准物。结果表明，钯（II）配合物作为有效的抗氧化剂具有很强的潜力，使其在食品和医药应用中具有重要价值。
