Greenberger J S, Weichselbaum R R, Botnick L E, Sakakeeny M, Moloney W C
Exp Hematol. 1979;7 Suppl 5:279-89.
Radiation therapy is used as total body treatment in preparation of the acute myelogenous leukemia (AML) patient for bone marrow transplantation. Many AML patients will have residual leukemia cells at the time of total body irradiation (TBI). In the present study, the effect of TBI on leukemic myeloid cells was compared to the effect on normal marrow granulocytic stem cells (CFUc) in vitro. Little difference from that of normal CFUc was found in the radiosensitivity of two mouse myeloid leukemia cell lines. The effect of TBI on growth of WEHI-3 or J774 cells in millipore diffusion chambers was stimulatory. These AML cell lines as well as others derived from Friend or Abelson virus infected in vitro long term mouse marrow cultures showed some morphologic differentiation by 7 days growth in diffusion chambers in irradiated heterologous rat hosts, but immature cells predominated by day 21. Thus, evidence in murine models of AML indicates that residual AML cells surviving chemotherapy will show no greater susceptibility to radiation killing compared to normal stem cells and will rapidly repopulate the irradiated host.
放射疗法被用作急性髓性白血病（AML）患者骨髓移植前的全身治疗。许多AML患者在全身照射（TBI）时会残留白血病细胞。在本研究中，体外比较了TBI对白血病髓细胞的影响与对正常骨髓粒细胞干细胞（CFUc）的影响。在两种小鼠髓性白血病细胞系的放射敏感性方面，未发现与正常CFUc有太大差异。TBI对微孔扩散室中WEHI-3或J774细胞生长的影响是刺激性的。这些AML细胞系以及其他源自体外感染Friend或Abelson病毒的长期小鼠骨髓培养物的细胞系，在照射的异种大鼠宿主的扩散室中生长7天时显示出一些形态学分化，但到第21天未成熟细胞仍占主导。因此，AML小鼠模型中的证据表明，与正常干细胞相比，化疗后存活的残留AML细胞对辐射杀伤的敏感性不会更高，并且会迅速在受照射宿主体内重新增殖。
