Zinc finger protein ZC3H18 is abnormally expressed in esophageal cancer tissues and facilitates the proliferation of esophageal cancer cells.

INTRODUCTION

Esophageal cancer presents significant challenges due to the limited efficacy and severe side effects associated with conventional treatments. The identification of epigenetic regulatory molecules that are aberrantly expressed in tumors is crucial for elucidating the mechanisms underlying the development and progression of esophageal cancer.

METHODS

We performed high-throughput methylation level analysis on cancerous and adjacent tissues from 25 patients, identifying the differentially methylated gene utilizing Bismark software and data from TCGA. Esophageal cancer cell lines with ZC3H18 knockdown were used to validate the biological role of ZC3H18 in tumorigenesis and . Eukaryotic transcriptome sequencing analysis was conducted to investigate the potential mechanisms underlying ZC3H18 function.

RESULTS

We identified 30 genes exhibiting significant methylation differences between cancerous and adjacent non-cancerous tissues in 25 patients. Subsequent analysis utilizing the TCGA database revealed that the gene is aberrantly expressed in tumor tissues and is closely associated with patient prognosis. Examination of esophageal cancer tissue samples demonstrated overexpression of the ZC3H18 protein, which was positively correlated with adverse prognosis indicators, including tumor differentiation, stage, and invasion depth. ZC3H18 knockdown significantly inhibited cellular proliferation, migration, invasion, and damage repair. Additionally, ZC3H18 significantly promoted tumor growth . The expression of various cytokeratins was significantly reduced following the gene knockdown. ZC3H18 and multiple keratins were co-localized in esophageal cancer tissue.

DISCUSSION

gene exhibits differential methylation in esophageal cancer was positively correlated with unfavorable patient prognosis. ZC3H18 plays a critical role in the regulation of biological functions within esophageal tumors.