Mallick Setu, Shormi Atia Shanjida, Jahan Hawa, Alam Mohammad Shamimul, Begum Rowshan Ara, Sarker Rakha Hari, Muid Khandaker Ashfaqul
Genetics and Molecular Biology branch, Department of Zoology, University of Dhaka, Dhaka, 1000, Bangladesh.
Department of Botany, University of Dhaka, Bangladesh.
Heliyon. 2025 Feb 20;11(4):e42898. doi: 10.1016/j.heliyon.2025.e42898. eCollection 2025 Feb 28.
Budding yeast, is an ideal model organism for genetic research due to its similarity in life cycle and cellular structure to higher eukaryotes as well as its ease of cultivation and manipulation in the laboratory. Yeast cells benefit from being cultured in calorie-restricted media, which can be achieved by reducing glucose concentration from 2 % to 0.5 %. Cell metabolism depends on glucose and therefore, affects the physiology of the cell. This study aimed to investigate the effects of long-term glucose starvation on the lifespan of yeast cells by culturing in both standard and glucose-starved conditions. In this investigation yeast cells (BY4743 strain) were cultured in glucose-restricted YPD media (0.5 percent dextrose) to assess lifespan, growth-proliferation, autophagy, apoptosis, mtDNA abundance. The findings revealed that prolonged glucose restriction significantly extended chronological lifespan in yeast (p < 0.05). In order to decipher how starved yeast live chronologically longer, we tested mitochondrial association and found that calorie deprivation lowered the rate of mtDNA spontaneous mutation and increased mtDNA abundance which is a suggestive sign of mitobiogenesis. Furthermore, cells cultured on glucose-restricted media led to more autophagosome formation but less cell death. These results suggested that glucose restriction can enhance lifespan by improving overall cellular conditions. These findings may serve as a foundation for future research in aging, cancer and diabetes.
出芽酵母因其生命周期和细胞结构与高等真核生物相似，以及在实验室易于培养和操作，是遗传研究的理想模式生物。酵母细胞在热量限制培养基中培养有益，这可通过将葡萄糖浓度从2%降至0.5%来实现。细胞代谢依赖葡萄糖，因此会影响细胞生理。本研究旨在通过在标准条件和葡萄糖饥饿条件下培养，研究长期葡萄糖饥饿对酵母细胞寿命的影响。在本研究中，酵母细胞（BY4743菌株）在葡萄糖限制的YPD培养基（0.5%葡萄糖）中培养，以评估寿命、生长增殖、自噬、凋亡、线粒体DNA丰度。研究结果显示，长期葡萄糖限制显著延长了酵母的时序寿命（p < 0.05）。为了解释饥饿酵母如何在时序上活得更长，我们测试了线粒体关联，发现热量剥夺降低了线粒体DNA自发突变率，并增加了线粒体DNA丰度，这是线粒体生物发生的一个暗示性迹象。此外，在葡萄糖限制培养基上培养的细胞导致更多自噬体形成，但细胞死亡减少。这些结果表明，葡萄糖限制可通过改善整体细胞状况来延长寿命。这些发现可能为未来衰老、癌症和糖尿病研究奠定基础。
