Preußner Mathieu, Bischoff Maik, Önel Susanne Filiz
Department of Biology, Developmental Biology, Philipps University Marburg, Karl-von-Frisch Str. 8, 35037 Marburg, Germany.
Department of Developmental Biology of Vertebrates, Institute of Cell Biology and Neuroscience, Goethe University Frankfurt am Main, 60438 Frankfurt am Main, Germany.
Cells. 2025 Mar 3;14(5):368. doi: 10.3390/cells14050368.
MicroRNAs function as post-transcriptional regulators in gene expression and control a broad range of biological processes in metazoans. The formation of multinucleated muscles is essential for locomotion, growth, and muscle repair. microRNAs have also emerged as important regulators for muscle development and function. In order to identify new microRNAs required for muscle formation, we have performed a large microRNA overexpression screen. We screened for defects during embryonic and adult muscle formation. Here, we describe the identification of mir-276a as a regulator for muscle migration during testis formation. The mir-276a overexpression phenotype in testis muscles resembles the loss-of-function phenotype of . A GFP sensor assay reveals that the 3'UTR of is a target of mir-276a. Furthermore, we found that mir-276a is essential for the proper development of indirect flight muscles and describe a method for determining the number of nuclei for each of the six longitudinal muscle fibers (DLMs), which are part of the indirect flight muscles.
微小RNA作为基因表达的转录后调节因子，控制后生动物广泛的生物学过程。多核肌肉的形成对于运动、生长和肌肉修复至关重要。微小RNA也已成为肌肉发育和功能的重要调节因子。为了鉴定肌肉形成所需的新微小RNA，我们进行了大规模的微小RNA过表达筛选。我们筛选了胚胎和成年肌肉形成过程中的缺陷。在此，我们描述了mir-276a作为睾丸形成过程中肌肉迁移调节因子的鉴定。睾丸肌肉中mir-276a的过表达表型类似于[此处缺失相关基因]的功能丧失表型。GFP传感器分析表明，[此处缺失相关基因]的3'UTR是mir-276a的靶标。此外，我们发现mir-276a对于间接飞行肌的正常发育至关重要，并描述了一种确定六条纵向肌纤维（DLM，间接飞行肌的一部分）中每条纤维细胞核数量的方法。
