蛋氨酸缺乏及再投喂期间大鼠肝脏的RNA聚合酶活性和染色质蛋白组成

RNA polymerase activities and chromatin protein composition of rat liver during methionine deprivation and refeeding.

作者信息

Norell M, von der Decken A

出版信息

Int J Biochem. 1985;17(3):325-30. doi: 10.1016/0020-711x(85)90207-1.

Abstract

The reversible effect of dietary methionine deficiency was studied in young adult rats. The sensitivity of nuclear chromatin to micrococcal nuclease (EC3.1.4.7) digestion and the composition of the chromatin proteins were unaffected by the dietary regimens. The specific chromatin-bound RNA polymerase II activity decreased during methionine deficiency. Refeeding of methionine for 2 days restored the activity in the nuclease-released chromatin. RNA polymerase I plus III activity remained unchanged. Total RNA polymerase activity changed with the liver wet weight which was reduced during methionine deficiency and was not restored to control level after 2 days of methionine refeeding. RNA polymerase activity was altered by methionine deficiency. The recovery was independent of major modifications of the chromatin structure and protein composition.

摘要

在年轻成年大鼠中研究了饮食中蛋氨酸缺乏的可逆效应。核染色质对微球菌核酸酶（EC3.1.4.7）消化的敏感性以及染色质蛋白的组成不受饮食方案的影响。在蛋氨酸缺乏期间，与染色质特异性结合的RNA聚合酶II活性降低。重新投喂蛋氨酸2天可恢复核酸酶释放的染色质中的活性。RNA聚合酶I加III的活性保持不变。总RNA聚合酶活性随肝脏湿重而变化，肝脏湿重在蛋氨酸缺乏期间降低，在重新投喂蛋氨酸2天后未恢复到对照水平。蛋氨酸缺乏会改变RNA聚合酶活性。这种恢复与染色质结构和蛋白质组成的主要改变无关。