Banko G, Zollner H
Int J Biochem. 1985;17(4):503-7. doi: 10.1016/0020-711x(85)90146-6.
The effect of ornithine on carbamoylphosphate formation of rat liver mitochondria treated with Triton X 100 was studied. The rate of carbamoylphosphate accumulation and citrulline formation depended on the ATP-, Pi-, N-acetylglutamate and protein concentration. At optimal conditions the rate of citrulline formation was at least 1.5-fold higher than the rate at which carbamoylphosphate accumulated (ornithine absent). A significant correlation between the amount of carbamoylphosphate formed and the citrulline/carbamoylphosphate ratio (ornithine effect) was found. In mitochondria the presence of a carbamoylphosphate degrading enzyme could be demonstrated. This enzyme may be one factor for the differences in the rate of carbamoylphosphate accumulation and the rate of citrulline synthesis.
研究了鸟氨酸对用Triton X 100处理的大鼠肝脏线粒体中氨甲酰磷酸形成的影响。氨甲酰磷酸积累速率和瓜氨酸形成速率取决于ATP、Pi、N-乙酰谷氨酸和蛋白质浓度。在最佳条件下，瓜氨酸形成速率比（无鸟氨酸时）氨甲酰磷酸积累速率至少高1.5倍。发现形成的氨甲酰磷酸量与瓜氨酸/氨甲酰磷酸比率（鸟氨酸效应）之间存在显著相关性。在线粒体中可证明存在一种氨甲酰磷酸降解酶。该酶可能是氨甲酰磷酸积累速率和瓜氨酸合成速率差异的一个因素。
