LaBonne S G, Mahowald A P
Dev Biol. 1985 Jul;110(1):264-7. doi: 10.1016/0012-1606(85)90084-3.
We show that embryos produced by Drosophila females homozygous for the maternal-effect neurogenic mutations almondex and pecanex can be partially rescued by microinjection of wild-type ooplasm. In addition, we demonstrate that these mutations fully complement one another with respect to this assay, as they do by classical genetic criteria. Finally, we show that embryos from both mutations cease to be rescuable after the establishment of the syncytial blastoderm. The significance of these results for our understanding of the neurogenic phenotype is discussed.
我们发现,对于母源性神经源性突变杏仁体(almondex)和山核桃体(pecanex)纯合的果蝇雌性所产生的胚胎,通过显微注射野生型卵质可以得到部分挽救。此外,我们证明,就该检测而言,这些突变彼此完全互补,就像它们在经典遗传学标准下那样。最后,我们表明,在合胞体胚盘形成后,来自这两种突变的胚胎就不再可挽救了。讨论了这些结果对于我们理解神经源性表型的意义。