Ikeda T, Ito Y, Murakami I, Mokuda O, Kuno S, Tokumori Y, Tominaga M, Mashiba H
Diabetes. 1985 Jul;34(7):647-52. doi: 10.2337/diab.34.7.647.
This study was undertaken to elucidate the effect of diabetes on the conversion of T4 to T3 and rT3 in the isolated, perfused rat liver and kidney. The livers and kidneys from streptozocin (STZ)-induced (50 mg/kg i.p. 2 wk before killing) diabetic rats with or without T4 (30 micrograms/kg s.c. daily) treatment were perfused for 30 min with a synthetic medium containing T4 (6 micrograms/dl), and production of T3 and rT3 in the tissues was measured by radioimmunoassay. The production of T3 (111 +/- 38 ng/g/30 min, mean +/- SD) and conversion rate of T4 to T3 (19.7 +/- 5.8%) in the liver of diabetic rats without T4 treatment and those (124 +/- 41 ng/g/30 min and 21.6 +/- 4.9%) in the liver of diabetic rats with T4 treatment were significantly lower than those of controls (196 +/- 48 ng/g/30 min and 30.6 +/- 5.2%), respectively. The production of rT3 and conversion rate of T4 to rT3 in the liver of diabetic rats with or without T4 treatment were similar to those of controls. The production of T3 and rT3, and conversion rate of T4 to T3 and T4 to rT3, in the kidney of diabetic rats with or without T4 treatment were not significantly different from those of controls. These results suggest that the liver is far more important than the kidney in the overall reduction in the T4 to T3 conversion that occurs in diabetic rats.
本研究旨在阐明糖尿病对离体灌注大鼠肝脏和肾脏中T4向T3及反T3转化的影响。将链脲佐菌素（STZ）诱导的糖尿病大鼠（处死前2周腹腔注射50mg/kg）的肝脏和肾脏，分为接受或未接受T4（每日皮下注射30μg/kg）治疗两组，用含T4（6μg/dl）的合成培养基灌注30分钟，通过放射免疫分析法测定组织中T3和反T3的生成量。未接受T4治疗的糖尿病大鼠肝脏中T3的生成量（111±38ng/g/30分钟，均值±标准差）及T4向T3的转化率（19.7±5.8%），和接受T4治疗的糖尿病大鼠肝脏中相应的值（124±41ng/g/30分钟和21.6±4.9%），均显著低于对照组（分别为196±48ng/g/30分钟和30.6±5.2%）。接受或未接受T4治疗的糖尿病大鼠肝脏中反T3的生成量及T4向反T3的转化率与对照组相似。接受或未接受T4治疗的糖尿病大鼠肾脏中T3和反T3的生成量，以及T4向T3和T4向反T3的转化率与对照组相比均无显著差异。这些结果表明，在糖尿病大鼠发生的T4向T3转化总体减少过程中，肝脏比肾脏重要得多。
