van der Sluis Renée M, García-Rodríguez Juan L, Nielsen Ian Helstrup, Gris-Oliver Albert, Becker Jennifer, Costa Bibiana, Chaudhry M Zeeshan, Werner Marvin, Laustsen Anders, Pedersen Jesper G, Gammelgaard Kristine R, Mogensen Trine H, Kalinke Ulrich, Cicin-Sain Luka, Bak Rasmus O, Kristensen Lasse S, Jakobsen Martin R
Aarhus Institute of Advanced Studies, Aarhus University, 8000 Aarhus, Denmark; Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Cell Rep. 2025 Mar 25;44(3):115413. doi: 10.1016/j.celrep.2025.115413. Epub 2025 Mar 11.
Plasmacytoid dendritic cells (pDCs) play a pivotal role in immune responses, particularly against viral infections. pDCs exhibit diverse functions, including interferon production, cytokine secretion, and antigen presentation. Here, we investigate the antigen cross-presentation capacity of pDCs and their role in CD8 T cell activation. Utilizing a culturing system with CD8 T cells and autologous pDCs derived from circulating CD34 hematopoietic stem and progenitor cells, we demonstrate that pDCs efficiently activate CD8 T cells via cross-presentation, promoting T cell expansion and cytotoxic activity. The antigen presentation capacity of pDCs is comparable to that of monocyte-derived dendritic cells (moDCs) and myeloid dendritic cells, which are known for their efficient antigen-presentation capacity. Transcriptomic analysis reveals genetic signatures in CD8 T cells activated by pDCs distinct from moDCs, suggesting different activation mechanisms. These findings underscore the importance of pDCs in antigen presentation and their contribution to CD8 T cell activation.
浆细胞样树突状细胞（pDCs）在免疫反应中发挥关键作用，尤其是在对抗病毒感染方面。pDCs具有多种功能，包括干扰素产生、细胞因子分泌和抗原呈递。在此，我们研究了pDCs的抗原交叉呈递能力及其在CD8 T细胞活化中的作用。利用含有CD8 T细胞和源自循环CD34造血干细胞及祖细胞的自体pDCs的培养系统，我们证明pDCs通过交叉呈递有效地激活CD8 T细胞，促进T细胞扩增和细胞毒性活性。pDCs的抗原呈递能力与以高效抗原呈递能力而闻名的单核细胞衍生树突状细胞（moDCs）和髓样树突状细胞相当。转录组分析揭示了由pDCs激活的CD8 T细胞中不同于moDCs的基因特征，表明存在不同的激活机制。这些发现强调了pDCs在抗原呈递中的重要性及其对CD8 T细胞活化的贡献。
