Kasi Phanindra Babu, Opoku Henry, Novikova Liudmila N, Wiberg Mikael, Kingham Paul J, Wang Jia, Novikov Lev N
Department of Medical and Translational Biology, Umeå University, Umeå SE-901 87, Sweden.
The Organic Photonics and Electronics Group, Department of Physics, Umeå University, Umeå SE-901 87, Sweden.
Colloids Surf B Biointerfaces. 2025 Jul;251:114609. doi: 10.1016/j.colsurfb.2025.114609. Epub 2025 Mar 7.
Quercetin, a flavonoid known for its antioxidant properties, has recently garnered attention as a potential neuroprotective agent for treatment of the injured nervous system. The repair of peripheral nerve injuries hinges on the proliferation and migration of Schwann cells, which play a crucial role in supporting axonal growth and myelination. In this study we synthesized Quercetin-derived carbon dots (QCDs) and investigated their effects on cultured Schwann cells and the NG108-15 cell line. QCDs was obtained by solvothermal synthesis and characterized via UV-vis absorption spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The particles demonstrated significant dose-dependent free radical scavenging activity in DPPH and ABTS radical scavenging assays, supported in vitro proliferation and migration of Schwann cells, expression of neurotrophic and angiogenic growth factors, and stimulated neurite outgrowth from NG108-15 cells. Thus, QCDs could serve as a potential novel treatment strategy to promote regeneration in the injured peripheral nervous system.
槲皮素是一种以其抗氧化特性而闻名的黄酮类化合物，最近作为一种治疗受损神经系统的潜在神经保护剂受到关注。周围神经损伤的修复取决于雪旺细胞的增殖和迁移，雪旺细胞在支持轴突生长和髓鞘形成中起关键作用。在本研究中，我们合成了槲皮素衍生的碳点（QCDs），并研究了它们对培养的雪旺细胞和NG108-15细胞系的影响。通过溶剂热合成获得QCDs，并通过紫外可见吸收光谱、透射电子显微镜、傅里叶变换红外光谱和X射线衍射分析对其进行表征。在DPPH和ABTS自由基清除试验中，这些颗粒表现出显著的剂量依赖性自由基清除活性，支持雪旺细胞的体外增殖和迁移、神经营养和血管生成生长因子的表达，并刺激NG108-15细胞的神经突生长。因此，QCDs可作为一种潜在的新型治疗策略，以促进受损周围神经系统的再生。
