Alrashdi Barakat M, Askar Hussam, Germoush Mousa O, Fouda Maged, Massoud Diaa, Alzwain Sarah, Abdelsater Naser, Salim Laila M S, Gadelmawla Mohamed H A, Ashry Mahmoud
Biology Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi Arabia.
Zoology Department, Faculty of Science, Al-Azhar University, 71524 Assiut, Egypt.
J Genet Eng Biotechnol. 2025 Mar;23(1):100466. doi: 10.1016/j.jgeb.2025.100466. Epub 2025 Feb 21.
Due to bleomycin's cytotoxic characteristics, which include cardiotoxicity, this investigation looked at the effectiveness of costus ethanolic extract in reducing cardiotoxicity in male rats receiving bleomycin therapy. Forty adult male rats (160-200 g) were evenly allocated into four groups: group (1) included normal rats serving as the control; group (2) included normal rats administered 200 mg/kg of costus ethanolic extract (CEE) orally for 6 weeks; group (3) consisted of rats receiving bleomycin (15 mg/kg twice weekly, ip) for 6 weeks; and group (4) involved rats treated orally with CEE (200 mg/kg/day) for 6 weeks following bleomycin intoxication. The results indicated that the CEE significantly reversed the cardiological deteriorations brought on by bleomycin; this was demonstrated by a considerable increase in cardiac SOD, GPx, GSH, and CAT, along with a substantial decrease in cardiac MDA, NO, and DNA fragmentation. Also, serum, LDH, CK-MB, CK- total, TNF-α, IL-4, IL-6 IL-10, IL-1β, triglycerides, cholesterol, and LDL were significantly reduced, while CD4 levels increased, and HDL declined significantly. The results of the histological and immunohistochemical analyses revealed a notable regeneration. In conclusion, CEE's anti-cardiotoxic, anti-inflammatory, and antioxidant properties prove its ability to be a cardio-protective supplement. This may be mediated by its active constituents' radical scavenging and antioxidant properties, particularly high phenolic content.
由于博来霉素具有细胞毒性特征，其中包括心脏毒性，本研究考察了闭鞘姜乙醇提取物在减轻接受博来霉素治疗的雄性大鼠心脏毒性方面的有效性。40只成年雄性大鼠（体重160 - 200克）被平均分为四组：第（1）组为正常大鼠作为对照组；第（2）组为正常大鼠，口服200毫克/千克闭鞘姜乙醇提取物（CEE），持续6周；第（3）组由接受博来霉素（15毫克/千克，每周两次，腹腔注射）治疗6周的大鼠组成；第（4）组为在博来霉素中毒后口服CEE（200毫克/千克/天）治疗6周的大鼠。结果表明，CEE显著逆转了博来霉素引起的心脏功能恶化；这表现为心脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽（GSH）和过氧化氢酶（CAT）显著增加，同时心脏丙二醛（MDA）、一氧化氮（NO）和DNA片段化显著减少。此外，血清乳酸脱氢酶（LDH）、肌酸激酶同工酶（CK - MB）、总肌酸激酶（CK - total）、肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 4（IL - 4）、白细胞介素 - 6（IL - 6）、白细胞介素 - 10（IL - 10）、白细胞介素 - 1β（IL - 1β）、甘油三酯、胆固醇和低密度脂蛋白（LDL）显著降低，而CD4水平升高，高密度脂蛋白（HDL）显著下降。组织学和免疫组织化学分析结果显示有明显的再生。总之，CEE的抗心脏毒性、抗炎和抗氧化特性证明了其作为心脏保护补充剂的能力。这可能是由其活性成分的自由基清除和抗氧化特性介导的，特别是高酚含量。
