揭示分层氧化石墨烯在一氧化氮清除中的作用。

Unveiling the Role of Fractionated Graphene Oxide in Nitric Oxide Scavenging.

作者信息

Chermashentsev Grigoriy R, Mikheev Ivan V, Ratova Daria-Mariia V, Proskurnina Elena V, Proskurnin Mikhail A

机构信息

Department of Chemistry, Lomonosov Moscow State University, Moscow 119234, Russia.

Research Centre for Medical Genetics, Moscow 115522, Russia.

出版信息

Molecules. 2025 Feb 26;30(5):1069. doi: 10.3390/molecules30051069.

DOI:10.3390/molecules30051069

PMID:40076294

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11901896/

Abstract

The feasibility of saturating aqueous anoxic solutions with in situ-generated high-purity nitric oxide (NO) is shown herein. A methemoglobin assay estimated the average nitric oxide concentration to be ca. 20 ± 3 µM. Graphene oxide aqueous dispersions were prepared by ultrasound-assisted extra exfoliation. These dispersions, including unpurified (pristine) samples and samples purified from transition metal impurities (bulk) fractions (bulkGO) and (nano) separated fractions (nanoGO) in a range of 0.5 to 14 kDa were prepared with ppm level concentrations. A robust and reproducible chemiluminescence (CL) assay validated the interaction between graphene oxide and NO in a luminol-based system. The results showed a significant increase in NO scavenging activity within the bulkGO fractions to nanofractions ranging from 14 to 3.5 kDa. The different reaction pathways underlying the transformation of nitric oxide are being evaluated, focusing on understanding how its presence or absence affects these processes. Our kinetic model suggests a significant difference in nitric oxide regulation; nanoGO demonstrates an interception rate seventy-times higher than that achieved through CL quenching.

摘要

本文展示了用原位生成的高纯度一氧化氮（NO）使缺氧水溶液饱和的可行性。高铁血红蛋白测定法估计一氧化氮的平均浓度约为20±3μM。通过超声辅助额外剥离制备氧化石墨烯水分散体。这些分散体包括未纯化（原始）样品以及从过渡金属杂质中纯化的样品，制备了浓度为ppm级的0.5至14 kDa范围内的（本体）级分（本体氧化石墨烯）和（纳米）分离级分（纳米氧化石墨烯）。一种稳健且可重现的化学发光（CL）测定法验证了在基于鲁米诺的体系中氧化石墨烯与NO之间的相互作用。结果表明，从14 kDa到3.5 kDa的本体氧化石墨烯级分到纳米级分中，NO清除活性显著增加。正在评估一氧化氮转化背后的不同反应途径，重点是了解其存在与否如何影响这些过程。我们的动力学模型表明一氧化氮调节存在显著差异；纳米氧化石墨烯的拦截率比通过CL猝灭实现的拦截率高七十倍。