Ahmed Waqar, Wang Yan, Ji Wenxia, Liu Songsong, Zhou Shun, Pan Jidong, Li Zhiguang, Wang Fusheng, Wang Xinrong
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2025 Feb 23;26(5):1922. doi: 10.3390/ijms26051922.
Endophytic species from agricultural crops have been extensively studied for their plant-growth-promoting (PGP) potential, but little is known about their PGP potential when isolated from perennial trees. This study investigated the plant-growth-promoting (PGP) potential of an endophyte, GDW1, isolated from a healthy pine tree by taking tomato as a host plant. We employed multiomics approaches (transcriptome and bacteriome analyses) to elucidate the underlying PGP mechanisms of GDW1. The results of greenhouse experiments revealed that the application of GDW1 significantly improved tomato plant growth, increasing shoot length, root length, fresh weight, and biomass accumulation by up to 44%, 38%, 54%, and 59%, respectively, compared with control. Transcriptomic analysis revealed 1158 differentially expressed genes significantly enriched in the plant hormone signaling (auxin, gibberellin, and cytokinin) and stress response (plant-pathogen interaction, MAPK signaling pathway-plant, and phenylpropanoid biosynthesis) pathways. Protein-protein interaction network analysis revealed nine hub genes (, , , , , , , , and ) related to stress tolerance, hormone control, and plant defense. Analysis of the tomato root bacteriome through 16S rRNA gene amplicon sequencing revealed that GDW1 inoculation dramatically altered the root bacterial community structure, enhancing the diversity and abundance of beneficial taxa (Proteobacteria and Bacteroidota). Co-occurrence network analysis showed a complex bacterial network in treated plants, suggesting increasingly intricate microbial relationships and improved nutrient absorption. Additionally, FAPROTAX and PICRUSt2 functional prediction analyses suggested the role of GDW1 in nitrogen cycling, organic matter degradation, plant growth promotion, and stress resistance. In conclusion, this study provides novel insights into the symbiotic relationship between GDW1 and tomato plants, highlighting its potential as a biofertilizer for sustainable agriculture and a means of reducing the reliance on agrochemicals.
来自农作物的内生菌因其促进植物生长的潜力而受到广泛研究，但从多年生树木中分离出的内生菌的促生长潜力却鲜为人知。本研究以番茄为寄主植物，调查了从健康松树中分离出的内生菌GDW1的促植物生长潜力。我们采用多组学方法（转录组和细菌组分析）来阐明GDW1潜在的促植物生长机制。温室实验结果表明，与对照相比，施用GDW1显著促进了番茄植株的生长，茎长、根长、鲜重和生物量积累分别增加了44%、38%、54%和59%。转录组分析显示，1158个差异表达基因显著富集于植物激素信号传导（生长素、赤霉素和细胞分裂素）和应激反应（植物-病原体相互作用、植物丝裂原活化蛋白激酶信号通路和苯丙烷生物合成）途径。蛋白质-蛋白质相互作用网络分析揭示了9个与胁迫耐受性、激素调控和植物防御相关的枢纽基因（、、、、、、、和）。通过16S rRNA基因扩增子测序对番茄根细菌组进行分析，结果表明接种GDW1显著改变了根细菌群落结构，增加了有益类群（变形菌门和拟杆菌门）的多样性和丰度。共现网络分析显示处理过的植物中存在复杂的细菌网络，表明微生物关系日益复杂且养分吸收得到改善。此外，FAPROTAX和PICRUSt2功能预测分析表明GDW1在氮循环、有机物降解、植物生长促进和抗逆性方面的作用。总之，本研究为GDW1与番茄植株之间的共生关系提供了新的见解，突出了其作为可持续农业生物肥料以及减少对农用化学品依赖手段的潜力。
