Wang Min, Yang Tianming, Xiang Yongjing, Pang Junxiao, Wang Yao, Sun Dali
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, China.
College of Food Science and Engineering, Guiyang University, Guiyang 550005, China.
Foods. 2025 Feb 24;14(5):770. doi: 10.3390/foods14050770.
Hyperlipidemia is characterized by a high level of blood lipid which poses a serious threat to human health. seed is a traditional crop of medicine and food homology with a wide range of pharmacological actions. To make clear the attenuation effect of seed against hyperlipidemia, low and high doses of seed extract (CSE) were orally administered to hyperlipidemia model mice developed by high-fat diet (HFD). Our results showed that CSE notably improved liver pathological injury, and oxidative stress, and declined the levels of glucose and lipid in hyperlipidemia mice. Liver metabolomics showed that lipid-related metabolites notably decreased, and pathways of glycolipid metabolism were seriously affected by CSE intervention. Moreover, 16S rRNA sequencing revealed that CSE treatment notably increased the diversity of gut microbiota. Meanwhile, the microbiota with the function of regulating intestinal balance as well as relieving obesity and nervous diseases significantly enhanced while harmful flora notably decreased after CSE intervention. The results of network pharmacology and molecular docking indicated that the PPAR signaling pathway may be the core path of anti-hyperlipidemia for seeds. RT-qPCR further verified that the expression levels of genes from the PPAR pathway notably changed by CSE treatment with fat synthesis genes significantly decreased while lipolysis genes notably enhanced. Therefore, seed might be a potential candidate for the treatment of hyperlipidemia.
高脂血症的特征是血脂水平高，这对人类健康构成严重威胁。[种子名称]是一种药食同源的传统作物，具有广泛的药理作用。为了明确[种子名称]对高脂血症的缓解作用，将低剂量和高剂量的[种子名称]提取物（CSE）口服给予由高脂饮食（HFD）诱导的高脂血症模型小鼠。我们的结果表明，CSE显著改善了肝脏病理损伤和氧化应激，并降低了高脂血症小鼠的血糖和血脂水平。肝脏代谢组学显示，脂质相关代谢物显著减少，糖脂代谢途径受到CSE干预的严重影响。此外，16S rRNA测序显示，CSE处理显著增加了肠道微生物群的多样性。同时，CSE干预后，具有调节肠道平衡以及缓解肥胖和神经疾病功能的微生物群显著增加，而有害菌群显著减少。网络药理学和分子对接结果表明，PPAR信号通路可能是[种子名称]抗高脂血症的核心途径。RT-qPCR进一步证实，CSE处理显著改变了PPAR途径基因的表达水平，脂肪合成基因显著降低，而脂肪分解基因显著增强。因此，[种子名称]可能是治疗高脂血症的潜在候选药物。
