Chen Dongying, Bi Xin, Feng Qian, Sun Yong
The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, China.
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Foods. 2025 Mar 3;14(5):870. doi: 10.3390/foods14050870.
In this study, the impact of lentil hull soluble dietary fibers (SDFs) on colitis and behavioral deficits in mice was assessed. Structural characterizations of SDFs confirmed that cellulase-modified soluble dietary fiber exhibited better physicochemical properties: more porous microstructure; similar polysaccharide structure; more stable particle size distribution; higher crystallinity; better adsorption capacity; and lower viscosity. Additionally, we explored its potential cognitive benefits via the gut-brain axis by behavioral tests, histopathology, 16S rRNA sequencing, gas chromatography and metabolomics analysis. The results showed that SDFs significantly improved inflammatory symptoms in colon and brain and cognitive behaviors. LSDF had better efficacy than HSDF. LSDF intervention decreased the harmful bacteria abundance (, and , etc.) and increased beneficial bacteria abundance ( and , etc.). LSDF also affected brain metabolites through the sphingolipid metabolism. Spearman correlation analysis showed that there was a positive correlation between harmful bacteria with inflammatory factors (LPS, IL-1β, IL-6, and TNF-α, etc.) and sphingolipid metabolites, while beneficial bacteria were positively correlated with brain-derived neurotrophic factor (BDNF), IL-10, and cognitive behavior. This study highlights the value of SDFs in future diet-based therapeutic strategies targeting gut-brain interactions.
在本研究中，评估了小扁豆壳可溶性膳食纤维（SDFs）对小鼠结肠炎和行为缺陷的影响。SDFs的结构表征证实，纤维素酶改性可溶性膳食纤维表现出更好的物理化学性质：更多孔的微观结构；相似的多糖结构；更稳定的粒度分布；更高的结晶度；更好的吸附能力；以及更低的粘度。此外，我们通过行为测试、组织病理学、16S rRNA测序、气相色谱和代谢组学分析，通过肠-脑轴探索了其潜在的认知益处。结果表明，SDFs显著改善了结肠和大脑中的炎症症状以及认知行为。低分子量SDF（LSDF）比高分子量SDF（HSDF）具有更好的疗效。LSDF干预降低了有害细菌的丰度（如大肠杆菌、肠球菌等），并增加了有益细菌的丰度（如双歧杆菌、乳酸杆菌等）。LSDF还通过鞘脂代谢影响脑代谢物。Spearman相关性分析表明，有害细菌与炎症因子（LPS、IL-1β、IL-6和TNF-α等）和鞘脂代谢物之间存在正相关，而有益细菌与脑源性神经营养因子（BDNF）、IL-10和认知行为呈正相关。本研究突出了SDFs在未来针对肠-脑相互作用的基于饮食的治疗策略中的价值。
