Doherty P C, Bartke A, Smith M S, Davis S L
Horm Behav. 1985 Jun;19(2):111-21. doi: 10.1016/0018-506x(85)90012-1.
To determine if deficits in sexual activity observed in pituitary-grafted male rats are due to elevated serum prolactin (PRL) levels found in these animals, the effects of whole pituitary grafts, pars distalis grafts, and ovine (o) PRL treatment on male copulatory behavior were compared. Adult sexually experienced CDF male rats were given four whole pituitary grafts, four pars distalis grafts, or were sham operated. Both groups of grafted animals exhibited suppressed copulatory behavior patterns when tested 18 days after pituitary transplantation. Animals given whole pituitary grafts had significantly longer latencies to mount (P less than 0.05) and to intromit (P less than 0.01) than did the sham-operated controls, while the animals given anterior pituitary grafts differed from the sham-operated controls in latencies to mount (P less than 0.05) and to intromit (P less than 0.01), as well as in the number of intromissions (P less than 0.05). Prolactin-injected animals had significantly reduced intromission rates (P less than 0.01) and significantly increased latencies to mount (P less than 0.05) and to intromit (P less than 0.01) when compared to vehicle-injected controls. Furthermore, the time course of behavioral suppression was similar in oPRL-treated animals to that observed in pars distalis-grafted males, with both groups showing the onset of deficits in sexual activity within 8 to 9 days from the induction of the hyperprolactinemic state. The similarity in pattern and time to onset of behavioral suppression in pituitary-grafted and oPRL-treated animals suggests that behavioral deficits observed in animals with pituitary grafts result from chronic elevation of serum PRL levels.
为了确定垂体移植雄性大鼠中观察到的性行为缺陷是否归因于这些动物血清催乳素（PRL）水平升高，比较了全垂体移植、垂体远侧部移植和绵羊（o）PRL治疗对雄性交配行为的影响。成年有性经验的CDF雄性大鼠接受四个全垂体移植、四个垂体远侧部移植或进行假手术。两组移植动物在垂体移植后18天进行测试时，均表现出交配行为模式受到抑制。接受全垂体移植的动物与假手术对照组相比，骑跨潜伏期（P<0.05）和插入潜伏期（P<0.01）显著延长，而接受垂体前叶移植的动物与假手术对照组相比，骑跨潜伏期（P<0.05）、插入潜伏期（P<0.01）以及插入次数（P<0.05）均有差异。与注射赋形剂的对照组相比，注射催乳素的动物插入率显著降低（P<0.01），骑跨潜伏期（P<0.05）和插入潜伏期（P<0.01）显著延长。此外，oPRL治疗动物的行为抑制时间进程与垂体远侧部移植雄性动物中观察到的相似，两组在高催乳素血症状态诱导后8至9天内均出现性活动缺陷。垂体移植和oPRL治疗动物在行为抑制模式和开始时间上的相似性表明，垂体移植动物中观察到的行为缺陷是血清PRL水平慢性升高的结果。
