Li Yangshuo, Liang Jingyi, Li Weihuo, Jia Yixin, Yuan Sheng, Zhou Yuanyuan, Hou Yuee, Zhang Nan
School of Animal Science and Technology, Foshan University, Foshan, PR China.
Zhuhai Kerric Testing, Zhuhai, PR China.
Poult Sci. 2025 May;104(5):104974. doi: 10.1016/j.psj.2025.104974. Epub 2025 Mar 8.
Mycoplasma gallisepticum (MG) poses a significant threat to the goose breeding industry, with antimicrobial agents commonly employed for its treatment. However, the absence of standardized criteria for antimicrobial selection complicates clinical decision-making regarding the choice and dosage of these agents, contributing to the emergence of clinical resistance. In this study, we isolated 102 clinical strains of MG from geese in Guangdong. We determined the minimum inhibitory concentration (MIC) values for various antimicrobials frequently used in MG treatment, including danofloxacin, enrofloxacin, tilmicosin, tylosin, tylvalosin, valnemulin, tiamulin, and spectinomycin. After conducting a statistical analysis of the susceptibility test results, we employed ECOFFinder to establish the wild-type cutoff values (ECOFFs) for these antimicrobials against the MG isolates. The findings revealed a significant reduction in sensitivity among the clinical isolates to the eight tested antimicrobial agents. While valnemulin and tiamulin maintained relatively good sensitivity, enrofloxacin, danofloxacin, and spectinomycin exhibited notably diminished effectiveness. The determined ECOFFs for MG isolated from geese against the aforementioned antimicrobials were 3.2, 3.2, 0.8, 0.8, 0.08, 0.05, 0.05, and 3.2 μg/mL, respectively. This study is crucial for identifying resistant strains and investigating resistance mechanisms, in addition to providing a precise assessment of the resistance levels of MG isolated from geese in Guangdong. The insights gained from this research will serve as a valuable reference for the prevention and treatment of MG infections and will promote more effective clinical strategies regarding the use of antimicrobials.
鸡毒支原体（MG）对鹅养殖业构成重大威胁，治疗时通常使用抗菌药物。然而，缺乏抗菌药物选择的标准化标准使得临床在这些药物的选择和剂量决策方面变得复杂，进而导致临床耐药性的出现。在本研究中，我们从广东的鹅中分离出102株MG临床菌株。我们测定了MG治疗中常用的各种抗菌药物的最低抑菌浓度（MIC）值，包括达氟沙星、恩诺沙星、替米考星、泰乐菌素、泰万菌素、沃尼妙林、替米考星和壮观霉素。在对药敏试验结果进行统计分析后，我们使用ECOFFinder确定了这些抗菌药物针对MG分离株的野生型临界值（ECOFFs）。研究结果显示，临床分离株对这八种受试抗菌药物的敏感性显著降低。虽然沃尼妙林和替米考星保持了相对较好的敏感性，但恩诺沙星、达氟沙星和壮观霉素的有效性明显降低。从鹅中分离出的MG针对上述抗菌药物的测定ECOFFs分别为3.2、3.2、0.8、0.8、0.08、0.05、0.05和3.2μg/mL。本研究对于鉴定耐药菌株和研究耐药机制至关重要，此外还能对从广东鹅中分离出的MG的耐药水平进行精确评估。本研究获得的见解将为MG感染的预防和治疗提供有价值的参考，并将促进抗菌药物使用方面更有效的临床策略。
