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在二丙二醇介导的双相乳液体系中，利用超声处理破碎酵母细胞进行苯乙酰甲醇生物转化。

Phenylacetylcarbinol biotransformation by disrupted yeast cells using ultrasonic treatment in conjunction with a dipropylene glycol mediated biphasic emulsion system.

作者信息

Nunta Rojarej, Porninta Kritsadaporn, Sommanee Sumeth, Mahakuntha Chatchadaporn, Techapun Charin, Feng Juan, Htike Su Lwin, Khemacheewakul Julaluk, Phimolsiripol Yuthana, Jantanasakulwong Kittisak, Rachtanapun Pornchai, Bostong Usa, Kumar Anbarasu, Leksawasdi Noppol

机构信息

Center of Excellence, Agro-Bio-Circular-Green Industry (Agro-BCG) & Bioprocess Research Cluster (BRC), School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.

Division of Food Innovation and Business, Faculty of Agricultural Technology, Lampang Rajabhat University, Lampang, 52100, Thailand.

出版信息

Sci Rep. 2025 Mar 13;15(1):8722. doi: 10.1038/s41598-025-92947-0.

DOI:10.1038/s41598-025-92947-0
PMID:40082633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11906596/
Abstract

Biotransformation of a pharmaceutical precursor, phenylacetylcarbinol (PAC), could be achieved by frozen-thawed Candida tropicalis whole cells (FT-WHC). The treatment of FT-WHC, which contained intracellular pyruvate decarboxylase (PDC) enzyme, using high-power ultrasonication with varying amplitudes were compared with glass bead attrition (GBA) as well as control for the release of PDC. Ultrasonication at 20% amplitude (Ult20) proved to be the most effective, resulting in the highest volumetric and specific PDC activities of 0.210 ± 0.004 U/mL and 0.335 ± 0.033 U/mg protein, respectively. Disrupted FT-WHC using Ult20 exhibited a statistically significant (p ≤ 0.05) higher initial PAC production rate (3.26 ± 0.04 mM). The comparison of three organic phases, namely, vegetable oil (Vg-Oil), Vg-Oil + dipropylene glycol (DPG), and octanol in the two-phase emulsion system for PAC biotransformation revealed the highest statistically significant (p ≤ 0.05) overall PAC concentration of 28.9 ± 0.1 mM in Vg-Oil + DPG system. The novel addition of DPG helped facilitating the partitioning of PAC into aqueous phase, stabilizing specific PDC activity, and specific PAC productivity in combination with ultrasonication treatment.

摘要

药物前体苯乙酰甲醇（PAC）的生物转化可通过冻融的热带假丝酵母全细胞（FT-WHC）实现。将含有细胞内丙酮酸脱羧酶（PDC）的FT-WHC，采用不同振幅的高功率超声处理，并与玻璃珠研磨（GBA）以及用于释放PDC的对照进行比较。20%振幅的超声处理（Ult20）被证明是最有效的，分别产生了最高的体积PDC活性和比PDC活性，即0.210±0.004 U/mL和0.335±0.033 U/mg蛋白质。使用Ult20破坏的FT-WHC表现出统计学上显著更高（p≤0.05）的初始PAC产率（3.26±0.04 mM）。在用于PAC生物转化的两相乳液系统中，对三种有机相，即植物油（Vg-Oil）、Vg-Oil+二丙二醇（DPG）和辛醇进行比较，结果显示在Vg-Oil+DPG系统中，总体PAC浓度在统计学上显著最高（p≤0.05），为28.9±0.1 mM。新型添加物DPG有助于促进PAC在水相中的分配，结合超声处理稳定比PDC活性和比PAC生产率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/825b62f0c6ee/41598_2025_92947_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/aab92743e26a/41598_2025_92947_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/e7b0a2a9d3af/41598_2025_92947_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/f546146c7410/41598_2025_92947_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/f317263171aa/41598_2025_92947_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/820a2e1a1935/41598_2025_92947_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/a8498755f8da/41598_2025_92947_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/8d31176a77bf/41598_2025_92947_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/825b62f0c6ee/41598_2025_92947_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/aab92743e26a/41598_2025_92947_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/e7b0a2a9d3af/41598_2025_92947_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/f546146c7410/41598_2025_92947_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/f317263171aa/41598_2025_92947_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/820a2e1a1935/41598_2025_92947_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/a8498755f8da/41598_2025_92947_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/8d31176a77bf/41598_2025_92947_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/11906596/825b62f0c6ee/41598_2025_92947_Fig8_HTML.jpg

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