Hayashi H, Ohe Y, Hayashi T, Iwai K
J Biochem. 1985 Feb;97(2):441-8. doi: 10.1093/oxfordjournals.jbchem.a135078.
For the sequence analysis of histones rich in lysine, we modified the subprograms for two reagents of a JEOL JAS-47KS protein sequence analyzer. Together with this modification, the use of a synthetic carrier, Polybrene, the minimization of aldehyde contamination in Quadrol buffer, and the introduction of hydrophilic groups into epsilon-N-amino groups of lysine residues, markedly increased the repetitive yield of PTH-amino acids. Tetrahymena histones H3 and H4 were thus sequenced up to residues 104 and 92, respectively, in each consecutive analysis (Hayashi, T., Hayashi, H., Fusauchi, Y., & Iwai, K. (1984) J. Biochem. 95, 1741-1749; Hayashi, H., Nomoto, M., & Iwai, K. (1984) J. Biochem. 96, 1449-1456). The details for these improved procedures and results are described here.
对于富含赖氨酸的组蛋白进行序列分析时，我们对JEOL JAS - 47KS蛋白质序列分析仪的两种试剂的子程序进行了修改。伴随这一修改，使用合成载体聚凝胺、尽量减少Quadrol缓冲液中的醛污染以及将亲水性基团引入赖氨酸残基的ε - N - 氨基，显著提高了PTH - 氨基酸的重复产率。因此，在每次连续分析中，嗜热四膜虫组蛋白H3和H4分别被测序至第104位和第92位残基（林，T.，林，H.，舟内，Y.，& 岩井，K.（1984年）《生物化学杂志》95卷，1741 - 1749页；林，H.，野本，M.，& 岩井，K.（1984年）《生物化学杂志》96卷，1449 - 1456页）。此处描述了这些改进程序和结果的详细情况。
