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受体酪氨酸激酶CAD96CA和FGFR1作为昆虫保幼激素的细胞膜受体发挥作用。

Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone.

作者信息

Li Yan-Xue, Kang Xin-Le, Li Yan-Li, Wang Xiao-Pei, Yan Qiao, Wang Jin-Xing, Zhao Xiao-Fan

机构信息

Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Shandong, China.

出版信息

Elife. 2025 Mar 14;13:RP97189. doi: 10.7554/eLife.97189.

DOI:10.7554/eLife.97189
PMID:40085503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11908783/
Abstract

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran insect (cotton bollworm), a serious agricultural pest, as a model, we determined that receptor tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor receptor homologue (FGFR1) function as JH cell membrane receptors by their roles in JH-regulated gene expression, larval status maintaining, rapid intracellular calcium increase, phosphorylation of JH intracellular receptor MET1 and cofactor Taiman, and high affinity to JH III. Gene knockout of and by CRISPR/Cas9 in embryo and knockdown in various insect cells, and overexpression of CAD96CA and FGFR1 in mammalian HEK-293T cells all supported CAD96CA and FGFR1 transmitting JH signal as JH cell membrane receptors.

摘要

保幼激素（JH）对于维持昆虫幼虫状态很重要；然而，其细胞膜受体尚未被鉴定出来。我们以鳞翅目昆虫（棉铃虫，一种严重的农业害虫）为模型，确定受体酪氨酸激酶（RTKs）钙黏蛋白96ca（CAD96CA）和成纤维细胞生长因子受体同源物（FGFR1）作为JH细胞膜受体发挥作用，它们通过在JH调控的基因表达、幼虫状态维持、细胞内钙快速增加、JH细胞内受体MET1和辅因子Tai的磷酸化以及对JH III的高亲和力等方面发挥作用。通过CRISPR/Cas9在胚胎中对 和 进行基因敲除以及在各种昆虫细胞中进行敲低，以及在哺乳动物HEK - 293T细胞中过表达CAD96CA和FGFR1，所有这些都支持CAD96CA和FGFR1作为JH细胞膜受体传递JH信号。

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Sci Bull (Beijing). 2022 Jan 30;67(2):186-197. doi: 10.1016/j.scib.2021.06.019. Epub 2021 Jun 24.
2
Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach.表皮生长因子信号促进德国蟑螂中保幼激素的生物合成。
BMC Biol. 2022 Dec 13;20(1):278. doi: 10.1186/s12915-022-01484-z.
3
CRISPR/Cas9-mediated methoprene-tolerant 1 knockout results in precocious metamorphosis of beet armyworm (Spodoptera exigua) only at the late larval stage.
CRISPR/Cas9介导的耐甲氧普烯1基因敲除仅在甜菜夜蛾幼虫后期导致其早熟变态。
Insect Mol Biol. 2023 Apr;32(2):132-142. doi: 10.1111/imb.12819. Epub 2022 Dec 12.
4
Juvenile hormone promotes paracellular transport of yolk proteins via remodeling zonula adherens at tricellular junctions in the follicular epithelium.保幼激素通过重塑滤泡上皮细胞的桥粒连接处的紧密连接来促进卵黄蛋白的旁细胞运输。
PLoS Genet. 2022 Jun 27;18(6):e1010292. doi: 10.1371/journal.pgen.1010292. eCollection 2022 Jun.
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