Robinson J L, Tsimidou M, Macrae R
J Chromatogr. 1985 May 17;324(1):35-51. doi: 10.1016/s0021-9673(01)81306-6.
The mass detector, a light scattering detector for use with high-performance liquid chromatography systems, was studied in order to determine which factors might affect sensitivity and quantification in the determination of triglycerides and other lipid materials. The detector response was found to be non-linear for triglycerides. The sensitivity was not only affected by evaporation temperatures and atomiser inlet air pressures within the detector, but also by the mobile phase flow-rate, and its composition. Detection limits of less than 1 microgram were achieved for non-volatile, saturated triglycerides, by direct injection, and the reproducibility of responses was about +/- 6% with isocratic elution (excluding tributyrin which was too volatile to be quantified accurately), but somewhat greater (+/- 16%) with gradient elution.
对用于高效液相色谱系统的质量检测器（一种光散射检测器）进行了研究，以确定哪些因素可能会影响甘油三酯和其他脂质材料测定中的灵敏度和定量。发现该检测器对甘油三酯的响应呈非线性。灵敏度不仅受检测器内蒸发温度和雾化器入口空气压力的影响，还受流动相流速及其组成的影响。通过直接进样，对于非挥发性饱和甘油三酯，检测限可达到低于1微克，等度洗脱时响应的重现性约为±6%（不包括挥发性太强无法准确定量的三丁酸甘油酯），但梯度洗脱时重现性稍高（±16%）。
