Krupcík J, Hrivnák J
J Chromatogr Sci. 1976 Jan;14(1):4-17. doi: 10.1093/chromsci/14.1.4.
The principal aspects influencing analytical capillary gas chromatography of fatty acids up to C18 have been evaluated. Selected fundamental problems of interlaboratory exchange of retention data were problems of defined temperature in commercial thermostated air baths, of capillary tubing, and of stationary phases. A modification of commercial thermostats has been proposed in order to secure a defined temperature for glass capillary columns. It has been found that retention data of fatty-acid methyl esters can be measured under standard conditions with the same accuracy as retention data of hydrocarbons on squalane. Metal capillary columns coated with Apiezon L were found to be unsuitable for the analysis of fatty-acid methyl esters when compared with the results of their quantitative analysis in packed Apiezon L and polar capillary columns. Possibilities of a nontraditional statistical evaluation of the results of measurements are suggested. A program in FORTRAN IV language is given for the calculation of Kovats' retention indices for fatty-acid methyl esters.
已对影响C18及以下脂肪酸分析毛细管气相色谱的主要方面进行了评估。实验室间保留数据交换的一些基本问题包括商用恒温空气浴中定义温度的问题、毛细管柱的问题以及固定相的问题。为确保玻璃毛细管柱有定义的温度，已提出对商用恒温器进行改进。已发现，脂肪酸甲酯的保留数据可在标准条件下进行测量，其准确度与烃类在角鲨烷上的保留数据相同。与填充Apiezon L柱和极性毛细管柱中脂肪酸甲酯的定量分析结果相比，发现涂有Apiezon L的金属毛细管柱不适用于脂肪酸甲酯的分析。文中提出了对测量结果进行非传统统计评估的可能性。给出了一个用FORTRAN IV语言编写的程序，用于计算脂肪酸甲酯的科瓦茨保留指数。
